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10.1016/j.bioactmat.2020.07.003 http://scihub22266oqcxt.onion/10.1016/j.bioactmat.2020.07.003 C7355334!7355334!32691013     free     free     free Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 269.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Bioact+Mater 2020 ; 5 (4): 1053-61 Nephropedia Template TP {{tp|p=32691013|t=2020. Efficient hepatic delivery and protein expression enabled by optimized mRNA and ionizable lipid nanoparticle |pdf=|usr=}}{{32691013}} gab.com Text Efficient hepatic delivery and protein expression enabled by optimized mRNA and ionizable lipid nanoparticle JO: Bioact Mater http://www.kidney.de/pget.php?&tw=1&pmid=32691013 #FOAvip mRNA is a novel class of therapeutic modality that holds great promise in vaccination, protein replacement therapy, cancer immunotherapy, immune cell engineering etc. However, optimization of mRNA molecules and efficient in vivo delivery are quite important but challenging for its broad application. Here we present an ionizable lipid nanoparticle (iLNP) based on iBL0713 lipid for in vitro and in vivo expression of desired proteins using codon-optimized mRNAs. mRNAs encoding luciferase or erythropoietin (EPO) were prepared by in vitro transcription and formulated with proposed iLNP, to form iLP171/mRNA formulations. It was revealed that both luciferase and EPO proteins were successfully expressed by human hepatocellular carcinoma cells and hepatocytes. The maximum amount of protein expression was found at 6 h post-administration. The expression efficiency of EPO with codon-optimized mRNA was significantly higher than that of unoptimized mRNA. Moreover, no toxicity or immunogenicity was observed for these mRNA formulations. Therefore, our study provides a useful and promising platform for mRNA therapeutic development. Twit Text FOAVip Efficient hepatic delivery and protein expression enabled by optimized mRNA and ionizable lipid nanoparticle ????Bioact Mater http://www.kidney.de/pget.php?&tw=1&pmid=32691013 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=32691013 #FOAvip English Wikipedia <ref>{{Cite pmid|32691013}}</ref> Efficient hepatic delivery and protein expression enabled by optimized mRNA and ionizable lipid nanoparticle #MMPMID32691013 Yang T; Li C; Wang X; Zhao D; Zhang M; Cao H; Liang Z; Xiao H; Liang XJ; Weng Y; Huang Y Bioact Mater 2020[Dec]; 5 (4): 1053-61 PMID32691013show ga mRNA is a novel class of therapeutic modality that holds great promise in vaccination, protein replacement therapy, cancer immunotherapy, immune cell engineering etc. However, optimization of mRNA molecules and efficient in vivo delivery are quite important but challenging for its broad application. Here we present an ionizable lipid nanoparticle (iLNP) based on iBL0713 lipid for in vitro and in vivo expression of desired proteins using codon-optimized mRNAs. mRNAs encoding luciferase or erythropoietin (EPO) were prepared by in vitro transcription and formulated with proposed iLNP, to form iLP171/mRNA formulations. It was revealed that both luciferase and EPO proteins were successfully expressed by human hepatocellular carcinoma cells and hepatocytes. The maximum amount of protein expression was found at 6 h post-administration. The expression efficiency of EPO with codon-optimized mRNA was significantly higher than that of unoptimized mRNA. Moreover, no toxicity or immunogenicity was observed for these mRNA formulations. Therefore, our study provides a useful and promising platform for mRNA therapeutic development. äEfficient hepatic delivery and protein expression enabled by optimized(epmc).pdf DeepDyve Pubget Overpricing