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Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 J+Clin+Virol 2020 ; 129 (ä): 104541 Nephropedia Template TP
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Evaluation of the Aptima? transcription-mediated amplification assay (Hologic®) for detecting SARS-CoV-2 in clinical specimens #MMPMID32659713
J Clin Virol 2020[Aug]; 129 (ä): 104541 PMID32659713show ga
Background: The spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which appeared in late 2019, has been limited by isolating infected individuals. However, identifying such individuals requires accurate diagnostic tools. Objective: This study evaluates the capacity of the Aptima? Transcription-Mediated Amplification (TMA) assay (Hologic® Panther System) to detect the virus in clinical samples. Study design: We compared the Aptima? assay to two in-house real-time RT-PCR techniques, one running on the Panther Fusion? module and the other on the MagNA Pure 96 and Light-Cycler 480 instruments. We included a total of 200 respiratory specimens: 100 tested prospectively and 100 retrospectively (25 -ve/75 +ve). Results: The final Cohen?s kappa coefficients were: ??=?0.978 between the Aptima? and Panther Fusion? assays, ??=?0.945 between the Aptima? and MagNA/LC480 assays and ??=?0.956 between the MagNA/LC480 and Panther Fusion? assays. Conclusion: These findings indicate that the Aptima? SARS-CoV-2 TMA assay data agree well with those obtained with our routine methods and that this assay can be used to diagnose coronavirus disease 2019 (COVID-19).