Accumulation of antibiotic-resistant genes in anaerobic biofilm reactor fed with
household chemical products
#MMPMID32835161
Khuntia HK
; Chanakya HN
SN Appl Sci
2020[]; 2
(8
): 1320
PMID32835161
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This research aims to determine the presence of antibiotic-resistant genes (ARG)
in anaerobic biofilm reactors (ABR) fed with household chemical products (HCP)
such as laundry detergents and handwash without any influx of antibiotics. The
ABR comprised a three-chamber design with bottom sludge, a middle chamber
containing fluidized PVC spiral, and a top chamber with packed coir fiber as a
biofilm support medium, respectively. Four different ABRs were simultaneously
operated for a prolonged period (200 day) and subjected to variations in
physicochemical conditions. The ABRs fed with HCP exhibited solitary accumulation
of log (4.4-7.5) ermC gene copies/g VS whereas, ARG was undetectable in glucose
fed ABRs indicating that HCP exhibited antimicrobial activities synonyms to
Erythromycin. Accumulation of Erythromycin-C (ermC) was relatively higher on the
biofilm inhabiting PVC support medium and further accentuated by effluent
recycling to log 7.5 ermC gene copies at a ratio of ermC/16S gene copies of 0.65.
Physico-chemical factors such as substrate composition, biofilm support medium,
and effluent recycling simultaneously elevated the concentration of ermC genes.
The results indicated that HCP augments the accumulation of ARG in the
microbiome, subsequently, increasing the risk in ARG transmission from sewage
treatment plants to the ecology and humans.
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