Plasmonic/magnetic molybdenum trioxide and graphitic carbon nitride quantum
dots-based fluoroimmunosensing system for influenza virus
#MMPMID32834503
Achadu OJ
; Takemura K
; Khoris IM
; Park EY
Sens Actuators B Chem
2020[Oct]; 321
(?): 128494
PMID32834503
show ga
A novel magnetic/plasmonic-assisted fluoro-immunoassay system is developed for
the detection of influenza virus using magnetic-derivatized plasmonic molybdenum
trioxide quantum dots (MP-MoO(3) QDs) as the plasmonic/magnetic agent and
fluorescent graphitic carbon nitride quantum dots (gCNQDs) as the monitoring
probe. Specific antibody against influenza A virus was conjugated onto the
surface of MP-MoO(3) QDs and gCNQDs, respectively. In the presence of influenza A
virus (as the test virus), a core-satellite immunocomplex is formed between the
antibody-conjugated nanomaterials (Ab-MP-MoO(3) QDs and Ab-gCNQDs) and their
interaction resulted in the modulation and gradual enhancement of the
fluorescence intensity of the detection probe with the influenza virus
concentration-dependent increase. In addition, PL change without influenza A
virus was not observed. Limits of detection of 0.25 and 0.9?pg/mL were achieved
for Influenza virus A/New Caledonia (20/99/IVR/116) (H1N1) detection in deionized
water and human serum, respectively. Clinically isolated influenza virus
A/Yokohama (110/2009) (H3N2) was detected in the range of 45 - 25,000 PFU/mL,
with a limit of detection ca 45 PFU/mL (as opposed to a minimum of 5000 PFU/mL
for a commercial test kit). This developed biosensor provides a robust, sensitive
as well as a selective platform for influenza virus detection.
free
free
free
