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10.7554/eLife.55414 http://scihub22266oqcxt.onion/10.7554/eLife.55414 C7299349!7299349!32469311     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=32469311&cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       eLife 2020 ; 9 (ä): ä Nephropedia Template TP {{tp|p=32469311|t=2020. Genomic and phenotypic evolution of Escherichia coli in a novel citrate-only resource environment |pdf=|usr=}}{{32469311}} gab.com Text Genomic and phenotypic evolution of Escherichia coli in a novel citrate-only resource environment JO: eLife http://www.kidney.de/pget.php?&tw=1&pmid=32469311 #FOAvip Evolutionary innovations allow populations to colonize new ecological niches. We previously reported that aerobic growth on citrate (Cit+) evolved in an Escherichia coli population during adaptation to a minimal glucose medium containing citrate (DM25). Cit+ variants can also grow in citrate-only medium (DM0), a novel environment for E. coli. To study adaptation to this niche, we founded two sets of Cit+ populations and evolved them for 2500 generations in DM0 or DM25. The evolved lineages acquired numerous parallel mutations, many mediated by transposable elements. Several also evolved amplifications of regions containing the maeA gene. Unexpectedly, some evolved populations and clones show apparent declines in fitness. We also found evidence of substantial cell death in Cit+ clones. Our results thus demonstrate rapid trait refinement and adaptation to the new citrate niche, while also suggesting a recalcitrant mismatch between E. coli physiology and growth on citrate. Twit Text FOAVip Genomic and phenotypic evolution of Escherichia coli in a novel citrate-only resource environment ????eLife http://www.kidney.de/pget.php?&tw=1&pmid=32469311 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=32469311 #FOAvip English Wikipedia <ref>{{Cite pmid|32469311}}</ref> Genomic and phenotypic evolution of Escherichia coli in a novel citrate-only resource environment #MMPMID32469311 Blount ZD; Maddamsetti R; Grant NA; Ahmed ST; Jagdish T; Baxter JA; Sommerfeld BA; Tillman A; Moore J; Slonczewski JL; Barrick JE; Lenski RE eLife 2020[]; 9 (ä): ä PMID32469311show ga Evolutionary innovations allow populations to colonize new ecological niches. We previously reported that aerobic growth on citrate (Cit+) evolved in an Escherichia coli population during adaptation to a minimal glucose medium containing citrate (DM25). Cit+ variants can also grow in citrate-only medium (DM0), a novel environment for E. coli. To study adaptation to this niche, we founded two sets of Cit+ populations and evolved them for 2500 generations in DM0 or DM25. The evolved lineages acquired numerous parallel mutations, many mediated by transposable elements. Several also evolved amplifications of regions containing the maeA gene. Unexpectedly, some evolved populations and clones show apparent declines in fitness. We also found evidence of substantial cell death in Cit+ clones. Our results thus demonstrate rapid trait refinement and adaptation to the new citrate niche, while also suggesting a recalcitrant mismatch between E. coli physiology and growth on citrate. äGenomic and phenotypic evolution of Escherichia coli in a novel citrat(epmc).pdf DeepDyve Pubget Overpricing