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10.7554/eLife.50684 http://scihub22266oqcxt.onion/10.7554/eLife.50684 C7295575!7295575 !32484440     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=32484440 &cmd=llinks): Failed to open stream: HTTP request failed! 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Shear stress activates ADAM10 sheddase to regulate Notch1 via the Piezo1 force sensor in endothelial cells |pdf=|usr=}}{{32484440 }} gab.com Text Shear stress activates ADAM10 sheddase to regulate Notch1 via the Piezo1 force sensor in endothelial cells JO: Elife http://www.kidney.de/pget.php?&tw=1&pmid=32484440 #FOAvip Mechanical force is a determinant of Notch signalling but the mechanism of force detection and its coupling to Notch are unclear. We propose a role for Piezo1 channels, which are mechanically-activated non-selective cation channels. In cultured microvascular endothelial cells, Piezo1 channel activation by either shear stress or a chemical agonist Yoda1 activated a disintegrin and metalloproteinase domain-containing protein 10 (ADAM10), a Ca(2+)-regulated transmembrane sheddase that mediates S2 Notch1 cleavage. Consistent with this observation, we found Piezo1-dependent increase in the abundance of Notch1 intracellular domain (NICD) that depended on ADAM10 and the downstream S3 cleavage enzyme, ?-secretase. Conditional endothelial-specific disruption of Piezo1 in adult mice suppressed the expression of multiple Notch1 target genes in hepatic vasculature, suggesting constitutive functional importance in vivo. The data suggest that Piezo1 is a mechanism conferring force sensitivity on ADAM10 and Notch1 with downstream consequences for sustained activation of Notch1 target genes and potentially other processes. Twit Text FOAVip Shear stress activates ADAM10 sheddase to regulate Notch1 via the Piezo1 force sensor in endothelial cells ????Elife http://www.kidney.de/pget.php?&tw=1&pmid=32484440 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=32484440 #FOAvip English Wikipedia <ref>{{Cite pmid|32484440 }}</ref> Shear stress activates ADAM10 sheddase to regulate Notch1 via the Piezo1 force sensor in endothelial cells #MMPMID32484440 Caolo V ; Debant M ; Endesh N ; Futers TS ; Lichtenstein L ; Bartoli F ; Parsonage G ; Jones EA ; Beech DJ Elife 2020[Jun]; 9 (ä): ä PMID32484440 show ga Mechanical force is a determinant of Notch signalling but the mechanism of force detection and its coupling to Notch are unclear. We propose a role for Piezo1 channels, which are mechanically-activated non-selective cation channels. In cultured microvascular endothelial cells, Piezo1 channel activation by either shear stress or a chemical agonist Yoda1 activated a disintegrin and metalloproteinase domain-containing protein 10 (ADAM10), a Ca(2+)-regulated transmembrane sheddase that mediates S2 Notch1 cleavage. Consistent with this observation, we found Piezo1-dependent increase in the abundance of Notch1 intracellular domain (NICD) that depended on ADAM10 and the downstream S3 cleavage enzyme, ?-secretase. Conditional endothelial-specific disruption of Piezo1 in adult mice suppressed the expression of multiple Notch1 target genes in hepatic vasculature, suggesting constitutive functional importance in vivo. The data suggest that Piezo1 is a mechanism conferring force sensitivity on ADAM10 and Notch1 with downstream consequences for sustained activation of Notch1 target genes and potentially other processes. |ADAM10 Protein/*metabolism [MESH] |Amyloid Precursor Protein Secretases/*metabolism [MESH] |Animals [MESH] |Cells, Cultured [MESH] |Endothelial Cells/*metabolism [MESH] |Enzyme Activation [MESH] |Gene Expression Regulation [MESH] |Humans [MESH] |Ion Channels/antagonists & inhibitors/*metabolism [MESH] |Male [MESH] |Membrane Proteins/*metabolism [MESH] |Mice [MESH] |Mice, Inbred C57BL [MESH] |Protein Domains [MESH] |Receptor, Notch1/*metabolism [MESH] |Stress, Mechanical [MESH]Shear stress activates ADAM10 sheddase to regulate Notch1 via the Piez(epmc).pdf DeepDyve Pubget Overpricing