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10.1016/j.cca.2020.05.047 http://scihub22266oqcxt.onion/10.1016/j.cca.2020.05.047 C7278646!7278646 !32526218     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=32526218 &cmd=llinks): Failed to open stream: HTTP request failed! 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Evaluation of the EDI enzyme linked immunosorbent assays for the detection of SARS-CoV-2 IgM and IgG antibodies in human plasma |pdf=|usr=}}{{32526218 }} gab.com Text Evaluation of the EDI enzyme linked immunosorbent assays for the detection of SARS-CoV-2 IgM and IgG antibodies in human plasma JO: Clin Chim Acta http://www.kidney.de/pget.php?&tw=1&pmid=32526218 #FOAvip BACKGROUND: Besides SARS-CoV-2 RT-PCR testing, serological testing is emerging as additional option in COVID-19 diagnostics. Aim of this study was to evaluate novel immunoassays for detection of SARS-CoV-2 antibodies in human plasma. METHODS: Using EDI(TM) Novel Coronavirus COVID-19 Enzyme Linked Immunosorbent Assays (ELISAs), we measured SARS-CoV-2 IgM and IgG antibodies in 64 SARS-CoV-2 RT-PCR confirmed COVID-19 patients with serial blood samples (n = 104) collected at different time points from symptom onset. Blood samples from 200 healthy blood donors and 256 intensive care unit (ICU) patients collected before the COVID-19 outbreak were also used. RESULTS: The positivity rates in the COVID-19 patients were 5.9% for IgM and 2.9% for IgG ? 5 days after symptom onset; Between day 5 and day 10 the positivity rates were 37.1% for IgM and 37.1% for IgG and rose to 76.4% for IgM and 82.4% for IgG after > 10-15 days. After 15-22 days the "true" positivity rates were 94.4% for IgM and 100% for IgG. The "false" positivity rates were 0.5% for IgM and 1.0% for IgG in the healthy blood donors, 1.6% for IgM and 1.2% for IgG in ICU patients. CONCLUSIONS: This study shows high "true" vs. low "false" positivity rates for the EDI(TM) SARS-CoV-2 IgM and IgG ELISAs. Twit Text FOAVip Evaluation of the EDI enzyme linked immunosorbent assays for the detection of SARS-CoV-2 IgM and IgG antibodies in human plasma ????Clin Chim Acta http://www.kidney.de/pget.php?&tw=1&pmid=32526218 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=32526218 #FOAvip English Wikipedia <ref>{{Cite pmid|32526218 }}</ref> Evaluation of the EDI enzyme linked immunosorbent assays for the detection of SARS-CoV-2 IgM and IgG antibodies in human plasma #MMPMID32526218 Bundschuh C ; Egger M ; Wiesinger K ; Gabriel C ; Clodi M ; Mueller T ; Dieplinger B Clin Chim Acta 2020[Oct]; 509 (ä): 79-82 PMID32526218 show ga BACKGROUND: Besides SARS-CoV-2 RT-PCR testing, serological testing is emerging as additional option in COVID-19 diagnostics. Aim of this study was to evaluate novel immunoassays for detection of SARS-CoV-2 antibodies in human plasma. METHODS: Using EDI(TM) Novel Coronavirus COVID-19 Enzyme Linked Immunosorbent Assays (ELISAs), we measured SARS-CoV-2 IgM and IgG antibodies in 64 SARS-CoV-2 RT-PCR confirmed COVID-19 patients with serial blood samples (n = 104) collected at different time points from symptom onset. Blood samples from 200 healthy blood donors and 256 intensive care unit (ICU) patients collected before the COVID-19 outbreak were also used. RESULTS: The positivity rates in the COVID-19 patients were 5.9% for IgM and 2.9% for IgG ? 5 days after symptom onset; Between day 5 and day 10 the positivity rates were 37.1% for IgM and 37.1% for IgG and rose to 76.4% for IgM and 82.4% for IgG after > 10-15 days. After 15-22 days the "true" positivity rates were 94.4% for IgM and 100% for IgG. The "false" positivity rates were 0.5% for IgM and 1.0% for IgG in the healthy blood donors, 1.6% for IgM and 1.2% for IgG in ICU patients. CONCLUSIONS: This study shows high "true" vs. low "false" positivity rates for the EDI(TM) SARS-CoV-2 IgM and IgG ELISAs. |*Betacoronavirus/isolation & purification [MESH] |Aged [MESH] |Aged, 80 and over [MESH] |Antibodies, Viral/*blood [MESH] |COVID-19 [MESH] |Coronavirus Infections/*blood/diagnosis [MESH] |Enzyme-Linked Immunosorbent Assay/methods/standards [MESH] |Female [MESH] |Humans [MESH] |Immunoglobulin G/*blood [MESH] |Immunoglobulin M/*blood [MESH] |Male [MESH] |Middle Aged [MESH] |Pandemics [MESH] |Pneumonia, Viral/*blood/diagnosis [MESH] |SARS-CoV-2 [MESH]Evaluation of the EDI enzyme linked immunosorbent assays for the detec(epmc).pdf DeepDyve Pubget Overpricing