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10.1016/j.cca.2020.06.004 http://scihub22266oqcxt.onion/10.1016/j.cca.2020.06.004 C7272145!7272145 !32505774     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=32505774 &cmd=llinks): Failed to open stream: HTTP request failed! 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SARS-CoV-2 antibody characterization in emergency department, hospitalized and convalescent patients by two semi-quantitative immunoassays |pdf=|usr=}}{{32505774 }} gab.com Text SARS-CoV-2 antibody characterization in emergency department, hospitalized and convalescent patients by two semi-quantitative immunoassays JO: Clin Chim Acta http://www.kidney.de/pget.php?&tw=1&pmid=32505774 #FOAvip BACKGROUND: In the ongoing COVID-19 pandemic, there is an urgent need for comprehensive performance evaluation and clinical utility assessment of serological assays to understand the immune response to SARS-CoV-2. METHODS: IgM/IgG and total antibodies against SARS-CoV-2 were measured by a cyclic enhanced fluorescence assay (CEFA) and a microsphere immunoassay (MIA), respectively. Independent performance evaluation included imprecision, reproducibility, specificity and cross-reactivity (CEFA n = 320, MIA n = 364). Clinical utility was evaluated by both methods in 87 patients at initial emergency department visit, 28 during subsequent hospitalizations (106 serial samples), and 145 convalescent patients. Totally 916 patients and 994 samples were evaluated. RESULTS: Agreement of CEFA and MIA was 90.4%-94.5% (Kappa: 0.81-0.89) in 302 samples. CEFA and MIA detected SARS-CoV-2 antibodies in 26.2% and 26.3%, respectively, of ED patients. Detection rates increased over time reaching 100% after 21 days post-symptom onset. Longitudinal antibody kinetic changes by CEFA and MIA measurements correlated well and exhibited three types of seroconversion. Convalescent sera showed a wide range of antibody levels. CONCLUSION: Rigorously validated CEFA and MIA assays are reliable for detecting antibodies to SARS-CoV-2 and show promising clinical utility when evaluating immune response in hospitalized and convalescent patients, but are not useful for early screening at patient's initial ED visit. Twit Text FOAVip SARS-CoV-2 antibody characterization in emergency department, hospitalized and convalescent patients by two semi-quantitative immunoassays ????Clin Chim Acta http://www.kidney.de/pget.php?&tw=1&pmid=32505774 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=32505774 #FOAvip English Wikipedia <ref>{{Cite pmid|32505774 }}</ref> SARS-CoV-2 antibody characterization in emergency department, hospitalized and convalescent patients by two semi-quantitative immunoassays #MMPMID32505774 Yang HS ; Racine-Brzostek SE ; Lee WT ; Hunt D ; Yee J ; Chen Z ; Kubiak J ; Cantu M ; Hatem L ; Zhong E ; D'Ambrosio D ; Chadburn A ; Westblade L ; Glesby M ; Loda M ; Cushing MM ; Zhao Z Clin Chim Acta 2020[Oct]; 509 (ä): 117-125 PMID32505774 show ga BACKGROUND: In the ongoing COVID-19 pandemic, there is an urgent need for comprehensive performance evaluation and clinical utility assessment of serological assays to understand the immune response to SARS-CoV-2. METHODS: IgM/IgG and total antibodies against SARS-CoV-2 were measured by a cyclic enhanced fluorescence assay (CEFA) and a microsphere immunoassay (MIA), respectively. Independent performance evaluation included imprecision, reproducibility, specificity and cross-reactivity (CEFA n = 320, MIA n = 364). Clinical utility was evaluated by both methods in 87 patients at initial emergency department visit, 28 during subsequent hospitalizations (106 serial samples), and 145 convalescent patients. Totally 916 patients and 994 samples were evaluated. RESULTS: Agreement of CEFA and MIA was 90.4%-94.5% (Kappa: 0.81-0.89) in 302 samples. CEFA and MIA detected SARS-CoV-2 antibodies in 26.2% and 26.3%, respectively, of ED patients. Detection rates increased over time reaching 100% after 21 days post-symptom onset. Longitudinal antibody kinetic changes by CEFA and MIA measurements correlated well and exhibited three types of seroconversion. Convalescent sera showed a wide range of antibody levels. CONCLUSION: Rigorously validated CEFA and MIA assays are reliable for detecting antibodies to SARS-CoV-2 and show promising clinical utility when evaluating immune response in hospitalized and convalescent patients, but are not useful for early screening at patient's initial ED visit. |*Betacoronavirus/isolation & purification [MESH] |Adult [MESH] |Aged [MESH] |Aged, 80 and over [MESH] |Antibodies, Viral/*blood [MESH] |COVID-19 [MESH] |Clinical Laboratory Techniques/methods/*trends [MESH] |Cohort Studies [MESH] |Convalescence [MESH] |Coronavirus Infections/*blood/diagnosis/therapy [MESH] |Emergency Service, Hospital/*trends [MESH] |Female [MESH] |Hospitalization/*trends [MESH] |Humans [MESH] |Immunoassay/methods [MESH] |Longitudinal Studies [MESH] |Male [MESH] |Middle Aged [MESH] |Pandemics [MESH] |Pneumonia, Viral/*blood/diagnosis/therapy [MESH]SARS-CoV-2 antibody characterization in emergency department, hospital(epmc).pdf DeepDyve Pubget Overpricing