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10.1007/s41745-020-00178-1

http://scihub22266oqcxt.onion/10.1007/s41745-020-00178-1
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C7264973!7264973!32837038
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suck abstract from ncbi


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pmid32837038      J+Indian+Inst+Sci 2020 ; 100 (3): 579-88
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  • Single?Cell RNA Sequencing for Precision Oncology: Current State-of-Art #MMPMID32837038
  • Seow JJW; Wong RMM; Pai R; Sharma A
  • J Indian Inst Sci 2020[]; 100 (3): 579-88 PMID32837038show ga
  • Tumors exhibit genetic and phenotypic diversity leading to intra-tumor heterogeneity (ITH). Further complex ecosystem (stromal and immune cells) of tumors contributes into the ITH. This ITH allows tumors to overcome various selection pressures such as anti-cancer therapies and metastasis at distant organs. Single-cell RNA-seq (scRNA-seq) has provided unprecedented insights into ITH and its implications in drug resistance and metastasis. As scRNA-seq technology grows and provides many new findings, new tools on different programming platforms are frequently generated. Here, we aim to provide a framework and guidelines for new entrants into the field of scRNA-seq. In this review, we discuss the current state-of-art of scRNA-seq analysis step-by-step including filtering, normalization and analysis. First, we discuss the brief history of experimental methods, followed by data processing and implications in precision oncology.
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