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10.1007/s12250-020-00238-x http://scihub22266oqcxt.onion/10.1007/s12250-020-00238-x C7256182!7256182!32472451     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Virol+Sin 2020 ; 35 (5): 637-50 Nephropedia Template TP {{tp|p=32472451|t=2020. Baculovirus Surface Display of Zika Virus Envelope Protein Protects against Virus Challenge in Mouse Model |pdf=|usr=}}{{32472451}} gab.com Text Baculovirus Surface Display of Zika Virus Envelope Protein Protects against Virus Challenge in Mouse Model JO: Virol Sin http://www.kidney.de/pget.php?&tw=1&pmid=32472451 #FOAvip Zika virus (ZIKV) is emerging as a significant pathogen worldwide and may cause severe neurological disorders such as fetal microcephaly and Guillain-Barre syndrome. No drug or listed vaccines are currently available for preventing ZIKV infection. As a major target of neutralizing, ZIKV envelop (E) protein usually used for vaccine development. Nevertheless, the immunogenicity of ZIKV envelop (E) protein expressed by baculovirus display system has never been assessed. In this study, we reported a new strategy for surface display of ZIKV E protein by a recombinant baculovirus vector derived from Autographa californica multiple nuclear polyhedrosis virus (AcMNPV) and assessed its immunogenicity in mice. We produced recombinant fusion ZIKV E protein linked with signal peptide (SP) and transmembrane domain (TM) of AcMNPV GP64. The results showed that the recombinant protein was easy to produce by baculovirus display system. BALB/c mice immunized with this recombinant E protein developed ZIKV specific serum antibodies. The anti-E protein sera from the mice were able to effectively neutralize ZIKV in vitro. More importantly, AG6 (IFN-?/? and IFN-? receptor deficient) mice immunized with recombinant E protein were protected against lethal ZIKV challenge. Together, these findings demonstrated that the recombinant E protein displayed by baculovirus can be conveniently prepared and displayed good immunogenicity in immunized mice. It is a promising practical approach for prompting the development of vaccine and related immunology research.Electronic supplementary material: The online version of this article (10.1007/s12250-020-00238-x) contains supplementary material, which is available to authorized users. Twit Text FOAVip Baculovirus Surface Display of Zika Virus Envelope Protein Protects against Virus Challenge in Mouse Model ????Virol Sin http://www.kidney.de/pget.php?&tw=1&pmid=32472451 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=32472451 #FOAvip English Wikipedia <ref>{{Cite pmid|32472451}}</ref> Baculovirus Surface Display of Zika Virus Envelope Protein Protects against Virus Challenge in Mouse Model #MMPMID32472451 Luo D; Miao Y; Ke X; Tan Z; Hu C; Li P; Wang T; Zhang Y; Sun J; Liu Y; Wang H; Zheng Z Virol Sin 2020[Oct]; 35 (5): 637-50 PMID32472451show ga Zika virus (ZIKV) is emerging as a significant pathogen worldwide and may cause severe neurological disorders such as fetal microcephaly and Guillain-Barre syndrome. No drug or listed vaccines are currently available for preventing ZIKV infection. As a major target of neutralizing, ZIKV envelop (E) protein usually used for vaccine development. Nevertheless, the immunogenicity of ZIKV envelop (E) protein expressed by baculovirus display system has never been assessed. In this study, we reported a new strategy for surface display of ZIKV E protein by a recombinant baculovirus vector derived from Autographa californica multiple nuclear polyhedrosis virus (AcMNPV) and assessed its immunogenicity in mice. We produced recombinant fusion ZIKV E protein linked with signal peptide (SP) and transmembrane domain (TM) of AcMNPV GP64. The results showed that the recombinant protein was easy to produce by baculovirus display system. BALB/c mice immunized with this recombinant E protein developed ZIKV specific serum antibodies. The anti-E protein sera from the mice were able to effectively neutralize ZIKV in vitro. More importantly, AG6 (IFN-?/? and IFN-? receptor deficient) mice immunized with recombinant E protein were protected against lethal ZIKV challenge. Together, these findings demonstrated that the recombinant E protein displayed by baculovirus can be conveniently prepared and displayed good immunogenicity in immunized mice. It is a promising practical approach for prompting the development of vaccine and related immunology research.Electronic supplementary material: The online version of this article (10.1007/s12250-020-00238-x) contains supplementary material, which is available to authorized users. äBaculovirus Surface Display of Zika Virus Envelope Protein Protects ag(epmc).pdf DeepDyve Pubget Overpricing