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Deprecated: Implicit conversion from float 259.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Clin+Microbiol+Infect 2020 ; 26 (8): 1082-7 Nephropedia Template TP
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Diagnostic performance of seven rapid IgG/IgM antibody tests and the Euroimmun IgA/IgG ELISA in COVID-19 patients #MMPMID32473953
Van Elslande J; Houben E; Depypere M; Brackenier A; Desmet S; André E; Van Ranst M; Lagrou K; Vermeersch P
Clin Microbiol Infect 2020[Aug]; 26 (8): 1082-7 PMID32473953show ga
Objectives: To evaluate the diagnostic performance of seven rapid IgG/IgM tests and the Euroimmun IgA/IgG ELISA for antibodies against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in COVID-19 patients. Methods: Specificity was evaluated in 103 samples collected before January 2020. Sensitivity and time to seropositivity was evaluated in 167 samples from 94 patients with COVID-19 confirmed with RT-PCR on nasopharyngeal swab. Results: Specificity (confidence interval) of lateral flow assays (LFAs) was ?91.3% (84.0?95.5) for IgM, ?90.3% (82.9?94.8) for IgG, and ?85.4% (77.2?91.1) for the combination IgM OR IgG. Specificity of the ELISA was 96.1% (90.1?98.8) for IgG and only 73.8% (64.5?81.4) for IgA. Sensitivity 14?25 days after the onset of symptoms was between ?92.1% (78.5?98.0) and 100% (95.7?100) for IgG LFA compared to 89.5% (75.3?96.4) for IgG ELISA. Positivity of IgM OR IgG for LFA resulted in a decrease in specificity compared to IgG alone without a gain in diagnostic performance, except for VivaDiag. The results for IgM varied significantly between the LFAs with an average overall agreement of only 70% compared to 89% for IgG. The average dynamic trend to seropositivity for IgM was not shorter than for IgG. At the time of hospital admission the sensitivity of LFA was <60%. Conclusions: Sensitivity for the detection of IgG antibodies 14?25 days after the onset of symptoms was ?92.1% for all seven LFAs compared to 89.5% for the IgG ELISA. The results for IgM varied significantly, and including IgM antibodies in addition to IgG for the interpretation of LFAs did not improve the diagnostic performance.