The Concept of Making On-Chip Thermal Cycler for RT-PCR Using Conjugate Heat
Transfer in Diverging Microchannel
#MMPMID38624347
Duryodhan VS
; Singh SG
; Agrawal A
Trans Indian Natl Acad Eng
2020[]; 5
(2
): 221-223
PMID38624347
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Covid-19 is pandemic to which the world is fighting. Various precautionary
measures are being imposed all over the world which is affecting the routine life
of an individual and also the economy worldwide. Although, a definite vaccine is
still not known to medical science but they are able to distinguish Covid-19 from
the other types of flu. Presently this is being done by detecting the SARS-CoV-2
virus using RT-PCR technique as recommended by the World Health Organization
(WHO) (WHO, Geneva, 2020). Reverse Transcription Polymerase Chain Reaction
(RT-PCR) is a nucleic acid amplification test that converts the RNA into DNA and
subsequently amplifies the specific DNA targets. This method was already being
employed to detect the severe acute respiratory syndrome-associated coronavirus
(SARS-CoV) (Emery et al. in Emerg Infect Dis 10(2):311, 2004). The entire process
of DNA amplification takes place in three steps: denaturation, annealing, and
extension for which the sample is required to be maintained at constant
temperatures of 95, 55 and 72 °C, respectively. This article introduces the
technology to achieve a constant temperature which can be tweaked to develop
on-chip RT-PCR.
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