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10.1016/j.cmi.2020.05.009 http://scihub22266oqcxt.onion/10.1016/j.cmi.2020.05.009 C7243766!7243766 !32450255     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=32450255 &cmd=llinks): Failed to open stream: HTTP request failed! 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Severe acute respiratory syndrome coronavirus 2 RNA contamination of inanimate surfaces and virus viability in a health care emergency unit |pdf=|usr=}}{{32450255 }} gab.com Text Severe acute respiratory syndrome coronavirus 2 RNA contamination of inanimate surfaces and virus viability in a health care emergency unit JO: Clin Microbiol Infect http://www.kidney.de/pget.php?&tw=1&pmid=32450255 #FOAvip OBJECTIVES: To detect possible severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) RNA contamination of inanimate surfaces in areas at high risk of aerosol formation by patients with coronavirus disease 2019 (COVID-19). METHODS: Sampling was performed in the emergency unit and the sub-intensive care ward. SARS-CoV-2 RNA was extracted from swabbed surfaces and objects and subjected to real-time RT-PCR targeting RNA-dependent RNA polymerase and E genes. Virus isolation from positive samples was attempted in vitro on Vero E6 cells. RESULTS: Twenty-six samples were collected and only two were positive for low-level SARS-CoV-2 RNA, both collected on the external surface of continuous positive airway pressure helmets. All transport media were inoculated onto susceptible cells, but none induced a cytopathic effect on day 7 of culture. CONCLUSIONS: Even though daily contact with inanimate surfaces and patient fomites in contaminated areas may be a medium of infection, our data obtained in real-life conditions suggest that it might be less extensive than hitherto recognized. Twit Text FOAVip Severe acute respiratory syndrome coronavirus 2 RNA contamination of inanimate surfaces and virus viability in a health care emergency unit ????Clin Microbiol Infect http://www.kidney.de/pget.php?&tw=1&pmid=32450255 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=32450255 #FOAvip English Wikipedia <ref>{{Cite pmid|32450255 }}</ref> Severe acute respiratory syndrome coronavirus 2 RNA contamination of inanimate surfaces and virus viability in a health care emergency unit #MMPMID32450255 Colaneri M ; Seminari E ; Novati S ; Asperges E ; Biscarini S ; Piralla A ; Percivalle E ; Cassaniti I ; Baldanti F ; Bruno R ; Mondelli MU Clin Microbiol Infect 2020[Aug]; 26 (8 ): 1094.e1-1094.e5 PMID32450255 show ga OBJECTIVES: To detect possible severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) RNA contamination of inanimate surfaces in areas at high risk of aerosol formation by patients with coronavirus disease 2019 (COVID-19). METHODS: Sampling was performed in the emergency unit and the sub-intensive care ward. SARS-CoV-2 RNA was extracted from swabbed surfaces and objects and subjected to real-time RT-PCR targeting RNA-dependent RNA polymerase and E genes. Virus isolation from positive samples was attempted in vitro on Vero E6 cells. RESULTS: Twenty-six samples were collected and only two were positive for low-level SARS-CoV-2 RNA, both collected on the external surface of continuous positive airway pressure helmets. All transport media were inoculated onto susceptible cells, but none induced a cytopathic effect on day 7 of culture. CONCLUSIONS: Even though daily contact with inanimate surfaces and patient fomites in contaminated areas may be a medium of infection, our data obtained in real-life conditions suggest that it might be less extensive than hitherto recognized. |Animals [MESH] |Betacoronavirus/genetics/*growth & development [MESH] |Chlorocebus aethiops [MESH] |Coronavirus Envelope Proteins [MESH] |Equipment Contamination [MESH] |Fomites/*virology [MESH] |Humans [MESH] |Intensive Care Units [MESH] |Microbial Viability [MESH] |RNA-Dependent RNA Polymerase/*genetics [MESH] |Reverse Transcriptase Polymerase Chain Reaction [MESH] |SARS-CoV-2 [MESH] |Vero Cells [MESH] |Viral Envelope Proteins/*genetics [MESH]Severe acute respiratory syndrome coronavirus 2 RNA contamination of i(epmc).pdf DeepDyve Pubget Overpricing