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10.1093/infdis/jiaa243

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C7239108!7239108 !32382737
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suck abstract from ncbi


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pmid32382737
      J+Infect+Dis 2020 ; 222 (2 ): 189-193
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  • A Peptide-Based Magnetic Chemiluminescence Enzyme Immunoassay for Serological Diagnosis of Coronavirus Disease 2019 #MMPMID32382737
  • Cai XF ; Chen J ; Li Hu J ; Long QX ; Deng HJ ; Liu P ; Fan K ; Liao P ; Liu BZ ; Wu GC ; Chen YK ; Li ZJ ; Wang K ; Zhang XL ; Tian WG ; Xiang JL ; Du HX ; Wang J ; Hu Y ; Tang N ; Lin Y ; Ren JH ; Huang LY ; Wei J ; Gan CY ; Chen YM ; Gao QZ ; Chen AM ; He CL ; Wang DX ; Hu P ; Zhou FC ; Huang AL ; Wang DQ
  • J Infect Dis 2020[Jun]; 222 (2 ): 189-193 PMID32382737 show ga
  • BACKGROUND: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), a novel ?-coronavirus, causes severe pneumonia and has spread throughout the globe rapidly. The disease associated with SARS-CoV-2 infection is named coronavirus disease 2019 (COVID-19). To date, real-time reverse-transcription polymerase chain reaction (RT-PCR) is the only test able to confirm this infection. However, the accuracy of RT-PCR depends on several factors; variations in these factors might significantly lower the sensitivity of detection. METHODS: In this study, we developed a peptide-based luminescent immunoassay that detected immunoglobulin (Ig)G and IgM. The assay cutoff value was determined by evaluating the sera from healthy and infected patients for pathogens other than SARS-CoV-2. RESULTS: To evaluate assay performance, we detected IgG and IgM in the sera from confirmed patients. The positive rate of IgG and IgM was 71.4% and 57.2%, respectively. CONCLUSIONS: Therefore, combining our immunoassay with real-time RT-PCR might enhance the diagnostic accuracy of COVID-19.
  • |Adult [MESH]
  • |Antibodies, Viral/*blood [MESH]
  • |Betacoronavirus/*immunology [MESH]
  • |COVID-19 [MESH]
  • |COVID-19 Testing [MESH]
  • |COVID-19 Vaccines [MESH]
  • |Clinical Laboratory Techniques/*methods [MESH]
  • |Coronavirus Infections/*diagnosis/immunology [MESH]
  • |Female [MESH]
  • |Humans [MESH]
  • |Immunoenzyme Techniques/*methods [MESH]
  • |Immunoglobulin G/blood [MESH]
  • |Immunoglobulin M/blood [MESH]
  • |Luminescent Measurements [MESH]
  • |Male [MESH]
  • |Middle Aged [MESH]
  • |Pandemics [MESH]
  • |Peptides/immunology [MESH]
  • |Pneumonia, Viral/*diagnosis/immunology [MESH]
  • |Real-Time Polymerase Chain Reaction [MESH]
  • |Reverse Transcriptase Polymerase Chain Reaction [MESH]
  • |SARS-CoV-2 [MESH]
  • |Sensitivity and Specificity [MESH]
  • |Serologic Tests/*methods [MESH]


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