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10.1016/j.cmi.2020.05.001 http://scihub22266oqcxt.onion/10.1016/j.cmi.2020.05.001 C7227531!7227531 !32422408     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=32422408 &cmd=llinks): Failed to open stream: HTTP request failed! 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Saliva sample as a non-invasive specimen for the diagnosis of coronavirus disease 2019: a cross-sectional study |pdf=|usr=}}{{32422408 }} gab.com Text Saliva sample as a non-invasive specimen for the diagnosis of coronavirus disease 2019: a cross-sectional study JO: Clin Microbiol Infect http://www.kidney.de/pget.php?&tw=1&pmid=32422408 #FOAvip OBJECTIVES: Amid the increasing number of pandemic coronavirus disease 2019 (COVID-19) cases, there is a need for a quick and easy method to obtain a non-invasive sample for the detection of this novel coronavirus (severe acute respiratory syndrome coronavirus 2; SARS-CoV-2). We aimed to investigate the potential use of saliva samples as a non-invasive tool for the diagnosis of COVID-19. METHODS: From 27 March to 4 April 2020, we prospectively collected saliva samples and a standard nasopharyngeal and throat swab in persons seeking care at an acute respiratory infection clinic in a university hospital during the outbreak of COVID-19. Real-time polymerase chain reaction (RT-PCR) was performed, and the results of the two specimens were compared. RESULTS: Two-hundred pairs of samples were collected. Sixty-nine (34.5%) individuals were male, and the median (interquartile) age was 36 (28-48) years. Using nasopharyngeal and throat swab RT-PCR as the reference standard, the prevalence of COVID-19 diagnosed by nasopharyngeal and throat swab RT-PCR was 9.5%. The sensitivity and specificity of the saliva sample RT-PCR were 84.2% (95% CI 60.4%-96.6%), and 98.9% (95% CI 96.1%-99.9%), respectively. An analysis of the agreement between the two specimens demonstrated 97.5% observed agreement (? coefficient 0.851, 95% CI 0.723-0.979; p < 0.001). CONCLUSIONS: Saliva might be an alternative specimen for the diagnosis of COVID-19. The collection is non-invasive, and non-aerosol generating. This method could facilitate the diagnosis of the disease, given the simplicity of specimen collection and good diagnostic performance. Twit Text FOAVip Saliva sample as a non-invasive specimen for the diagnosis of coronavirus disease 2019: a cross-sectional study ????Clin Microbiol Infect http://www.kidney.de/pget.php?&tw=1&pmid=32422408 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=32422408 #FOAvip English Wikipedia <ref>{{Cite pmid|32422408 }}</ref> Saliva sample as a non-invasive specimen for the diagnosis of coronavirus disease 2019: a cross-sectional study #MMPMID32422408 Pasomsub E ; Watcharananan SP ; Boonyawat K ; Janchompoo P ; Wongtabtim G ; Suksuwan W ; Sungkanuparph S ; Phuphuakrat A Clin Microbiol Infect 2021[Feb]; 27 (2 ): 285.e1-285.e4 PMID32422408 show ga OBJECTIVES: Amid the increasing number of pandemic coronavirus disease 2019 (COVID-19) cases, there is a need for a quick and easy method to obtain a non-invasive sample for the detection of this novel coronavirus (severe acute respiratory syndrome coronavirus 2; SARS-CoV-2). We aimed to investigate the potential use of saliva samples as a non-invasive tool for the diagnosis of COVID-19. METHODS: From 27 March to 4 April 2020, we prospectively collected saliva samples and a standard nasopharyngeal and throat swab in persons seeking care at an acute respiratory infection clinic in a university hospital during the outbreak of COVID-19. Real-time polymerase chain reaction (RT-PCR) was performed, and the results of the two specimens were compared. RESULTS: Two-hundred pairs of samples were collected. Sixty-nine (34.5%) individuals were male, and the median (interquartile) age was 36 (28-48) years. Using nasopharyngeal and throat swab RT-PCR as the reference standard, the prevalence of COVID-19 diagnosed by nasopharyngeal and throat swab RT-PCR was 9.5%. The sensitivity and specificity of the saliva sample RT-PCR were 84.2% (95% CI 60.4%-96.6%), and 98.9% (95% CI 96.1%-99.9%), respectively. An analysis of the agreement between the two specimens demonstrated 97.5% observed agreement (? coefficient 0.851, 95% CI 0.723-0.979; p < 0.001). CONCLUSIONS: Saliva might be an alternative specimen for the diagnosis of COVID-19. The collection is non-invasive, and non-aerosol generating. This method could facilitate the diagnosis of the disease, given the simplicity of specimen collection and good diagnostic performance. |Adult [MESH] |COVID-19 Testing/*methods [MESH] |COVID-19/*diagnosis [MESH] |Cross-Sectional Studies [MESH] |Female [MESH] |Humans [MESH] |Male [MESH] |Middle Aged [MESH] |Nasopharynx/virology [MESH] |Prospective Studies [MESH] |RNA, Viral/analysis [MESH] |Real-Time Polymerase Chain Reaction [MESH] |SARS-CoV-2/*genetics [MESH] |Saliva/*virology [MESH] |Sensitivity and Specificity [MESH] |Specimen Handling/methods [MESH]Saliva sample as a non-invasive specimen for the diagnosis of coronavi(epmc).pdf DeepDyve Pubget Overpricing