A nanoparticle pseudo pathogen for rapid detection and diagnosis of virus
infection
#MMPMID34766034
Zheng T
; Huo Q
Sens Int
2020[]; 1
(?): 100010
PMID34766034
show ga
We herein report a new rapid blood test for virus infection detection and
diagnosis. A citrate gold nanoparticle is first coated with a virus lysate to
form a gold nanoparticle pseudo pathogen. The gold nanoparticle pseudo virus is
then mixed with a blood plasma or serum samples. If the blood sample is from a
positive patient, the activated immune molecules in the blood such as antibodies,
complement proteins and others will react with the nanoparticle pseudo virus,
leading to nanoparticle aggregate formation. The nanoparticle aggregate formation
is detected and measured using a particle sizing technique called dynamic light
scattering. In this study, we applied this test for Zika virus infection
detection. We tested blood plasma samples from 85 Zika positive patients, 40
Dengue positive patients, 10 Chikungunya positive patients, and 78 non-patient
control samples collected from both endemic and non-endemic locations. The study
shows that the new test has a higher sensitivity compared to some existing
commercial tests in the market, while maintaining a similar specificity. Within 7
days from the symptom onset, the new test can detect 43% of the infected patients
while a commercial anti-Zika IgM test detects only 26% of the infected patients.
Within 14 days from the symptom onset, our new test detects 73% of the infected
patients while the same commercial anti-Zika IgM test detects 53% of the infected
patients. The test is extremely simple, easy to develop, with test results
obtained within minutes. This new test platform may be potentially adapted for
the detection and diagnosis of a wide range of viral infectious diseases, for
example, the currently ongoing COVID-19.
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