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10.1093/ajcp/aqaa064 http://scihub22266oqcxt.onion/10.1093/ajcp/aqaa064 C7188150!7188150!32304208     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=32304208&cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Am+J+Clin+Pathol 2020 ; 153 (6): 715-8 Nephropedia Template TP {{tp|p=32304208|t=2020. Assessment of Specimen Pooling to Conserve SARS CoV-2 Testing Resources |pdf=|usr=}}{{32304208}} gab.com Text Assessment of Specimen Pooling to Conserve SARS CoV-2 Testing Resources JO: Am J Clin Pathol http://www.kidney.de/pget.php?&tw=1&pmid=32304208 #FOAvip Objectives: To establish the optimal parameters for group testing of pooled specimens for the detection of SARS-CoV-2. Methods: The most efficient pool size was determined to be five specimens using a web-based application. From this analysis, 25 experimental pools were created using 50 µL from one SARS-CoV-2 positive nasopharyngeal specimen mixed with 4 negative patient specimens (50 µL each) for a total volume of 250 µL. Viral RNA was subsequently extracted from each pool and tested using the CDC SARS-CoV-2 RT-PCR assay. Positive pools were consequently split into individual specimens and tested by extraction and PCR. This method was also tested on an unselected group of 60 nasopharyngeal specimens grouped into 12 pools. Results: All 25 pools were positive with cycle threshold (Ct) values within 0 and 5.03 Ct of the original individual specimens. The analysis of 60 specimens determined that 2 pools were positive followed by identification of 2 individual specimens among the 60 tested. This testing was accomplished while using 22 extractions/PCR tests, a savings of 38 reactions. Conclusions: When the incidence rate of SARS-CoV-2 infection is 10% or less, group testing will result in the saving of reagents and personnel time with an overall increase in testing capability of at least 69%. Twit Text FOAVip Assessment of Specimen Pooling to Conserve SARS CoV-2 Testing Resources ????Am J Clin Pathol http://www.kidney.de/pget.php?&tw=1&pmid=32304208 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=32304208 #FOAvip English Wikipedia <ref>{{Cite pmid|32304208}}</ref> Assessment of Specimen Pooling to Conserve SARS CoV-2 Testing Resources #MMPMID32304208 Abdalhamid B; Bilder CR; McCutchen EL; Hinrichs SH; Koepsell SA; Iwen PC Am J Clin Pathol 2020[May]; 153 (6): 715-8 PMID32304208show ga Objectives: To establish the optimal parameters for group testing of pooled specimens for the detection of SARS-CoV-2. Methods: The most efficient pool size was determined to be five specimens using a web-based application. From this analysis, 25 experimental pools were created using 50 µL from one SARS-CoV-2 positive nasopharyngeal specimen mixed with 4 negative patient specimens (50 µL each) for a total volume of 250 µL. Viral RNA was subsequently extracted from each pool and tested using the CDC SARS-CoV-2 RT-PCR assay. Positive pools were consequently split into individual specimens and tested by extraction and PCR. This method was also tested on an unselected group of 60 nasopharyngeal specimens grouped into 12 pools. Results: All 25 pools were positive with cycle threshold (Ct) values within 0 and 5.03 Ct of the original individual specimens. The analysis of 60 specimens determined that 2 pools were positive followed by identification of 2 individual specimens among the 60 tested. This testing was accomplished while using 22 extractions/PCR tests, a savings of 38 reactions. Conclusions: When the incidence rate of SARS-CoV-2 infection is 10% or less, group testing will result in the saving of reagents and personnel time with an overall increase in testing capability of at least 69%. äAssessment of Specimen Pooling to Conserve SARS CoV-2 Testing Resource(epmc).pdf DeepDyve Pubget Overpricing