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10.1002/pmic.201300001 http://scihub22266oqcxt.onion/10.1002/pmic.201300001 C7167617!7167617!24166946     free     free     free Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Proteomics 2013 ; 13 (23-24): 3442-56 Nephropedia Template TP {{tp|p=24166946|t=2013. Proteomic analysis for Type I interferon antagonism of Japanese encephalitis virus NS5 protein |pdf=|usr=}}{{24166946}} gab.com Text Proteomic analysis for Type I interferon antagonism of Japanese encephalitis virus NS5 protein JO: Proteomics http://www.kidney.de/pget.php?&tw=1&pmid=24166946 #FOAvip Japanese encephalitis virus (JEV) nonstructural protein 5 (NS5) exhibits a Type I interferon (IFN) antagonistic function. This study characterizes Type I IFN antagonism mechanism of NS5 protein, using proteomic approach. In human neuroblastoma cells, NS5 expression would suppress IFN??induced responses, for example, expression of IFN?stimulated genes PKR and OAS as well as STAT1 nuclear translocation and phosphorylation. Proteomic analysis showed JEV NS5 downregulating calreticulin, while upregulating cyclophilin A, HSP 60 and stress?induced?phosphoprotein 1. Gene silence of calreticulin raised intracellular Ca2+ levels while inhibiting nuclear translocalization of STAT1 and NFAT?1 in response to IFN?, thus, indicating calreticulin downregulation linked with Type I IFN antagonism of JEV NS5 via activation of Ca2+/calicineurin. Calcineurin inhibitor cyclosporin A attenuated NS5?mediated inhibition of IFN??induced responses, for example, IFN?sensitive response element driven luciferase, STAT1?dependent PKR mRNA expression, as well as phosphorylation and nuclear translocation of STAT1. Transfection with calcineurin (vs. control) siRNA enhanced nuclear translocalization of STAT1 and upregulated PKR expression in NS5?expressing cells in response to IFN?. Results prove Ca2+, calreticulin, and calcineurin involvement in STAT1?mediated signaling as well as a key role of JEV NS5 in Type I IFN antagonism. This study offers insights into the molecular mechanism of Type I interferon antagonism by JEV NS5. Twit Text FOAVip Proteomic analysis for Type I interferon antagonism of Japanese encephalitis virus NS5 protein ????Proteomics http://www.kidney.de/pget.php?&tw=1&pmid=24166946 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=24166946 #FOAvip English Wikipedia <ref>{{Cite pmid|24166946}}</ref> Proteomic analysis for Type I interferon antagonism of Japanese encephalitis virus NS5 protein #MMPMID24166946 Yang T; Li S; Lai C; Lu K; Chiu M; Hsieh T; Wan L; Lin C Proteomics 2013[Dec]; 13 (23-24): 3442-56 PMID24166946show ga Japanese encephalitis virus (JEV) nonstructural protein 5 (NS5) exhibits a Type I interferon (IFN) antagonistic function. This study characterizes Type I IFN antagonism mechanism of NS5 protein, using proteomic approach. In human neuroblastoma cells, NS5 expression would suppress IFN??induced responses, for example, expression of IFN?stimulated genes PKR and OAS as well as STAT1 nuclear translocation and phosphorylation. Proteomic analysis showed JEV NS5 downregulating calreticulin, while upregulating cyclophilin A, HSP 60 and stress?induced?phosphoprotein 1. Gene silence of calreticulin raised intracellular Ca2+ levels while inhibiting nuclear translocalization of STAT1 and NFAT?1 in response to IFN?, thus, indicating calreticulin downregulation linked with Type I IFN antagonism of JEV NS5 via activation of Ca2+/calicineurin. Calcineurin inhibitor cyclosporin A attenuated NS5?mediated inhibition of IFN??induced responses, for example, IFN?sensitive response element driven luciferase, STAT1?dependent PKR mRNA expression, as well as phosphorylation and nuclear translocation of STAT1. Transfection with calcineurin (vs. control) siRNA enhanced nuclear translocalization of STAT1 and upregulated PKR expression in NS5?expressing cells in response to IFN?. Results prove Ca2+, calreticulin, and calcineurin involvement in STAT1?mediated signaling as well as a key role of JEV NS5 in Type I IFN antagonism. This study offers insights into the molecular mechanism of Type I interferon antagonism by JEV NS5. äProteomic analysis for Type I interferon antagonism of Japanese enceph(epmc).pdf DeepDyve Pubget Overpricing