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10.1002/jmv.25350 http://scihub22266oqcxt.onion/10.1002/jmv.25350 C7167037!7167037!30358912     free     free     free Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 247.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 247.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 247.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 247.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 247.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 247.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Med+Virol 2019 ; 91 (4): 564-9 Nephropedia Template TP {{tp|p=30358912|t=2019. Detection of six common human paramyxoviruses in patients with acute febrile respiratory symptoms using a novel multiplex real?time RT?PCR assay |pdf=|usr=}}{{30358912}} gab.com Text Detection of six common human paramyxoviruses in patients with acute febrile respiratory symptoms using a novel multiplex real time RT PCR assay JO: J Med Virol http://www.kidney.de/pget.php?&tw=1&pmid=30358912 #FOAvip Human metapneumovirus (hMPV), respiratory syncytial virus type A (RSV?A), RSV?B, and human parainfluenza viruses 1, 2, and 3 (HPIV?1, HPIV?2, and HPIV?3) are common respiratory paramyxoviruses. Here, we developed a two?tube triplex one?step real?time reverse?transcription polymerase chain reaction (real?time RT?PCR) and evaluated its performance using clinical samples. The data showed that this novel assay was 100% consistent with the monoplex real?time RT?PCR assay (in?house), which was superior to the commercial routine multiplex?ligation?NAT?based assay. Meanwhile, the clinical nasopharyngeal swabs of 471 patients with the acute febrile respiratory syndrome (AFRS) were analyzed using the established method. The results showed that 52 (11.7%) cases were positive for paramyxovirus. Among them, HPIVs and RSV?A had the highest detection rate. The age and seasonal distribution of human paramyxovirus infection were analyzed. In conclusion, we developed a novel multiplex real?time RT?PCR assay for the rapid detection of six common human paramyxoviruses, which were dominant in patients with AFRS in Qinghai. Twit Text FOAVip Detection of six common human paramyxoviruses in patients with acute febrile respiratory symptoms using a novel multiplex real time RT PCR assay ????J Med Virol http://www.kidney.de/pget.php?&tw=1&pmid=30358912 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=30358912 #FOAvip English Wikipedia <ref>{{Cite pmid|30358912}}</ref> Detection of six common human paramyxoviruses in patients with acute febrile respiratory symptoms using a novel multiplex real?time RT?PCR assay #MMPMID30358912 Liu G; Niu P; Zhao S; Lu R; Tan W J Med Virol 2019[Apr]; 91 (4): 564-9 PMID30358912show ga Human metapneumovirus (hMPV), respiratory syncytial virus type A (RSV?A), RSV?B, and human parainfluenza viruses 1, 2, and 3 (HPIV?1, HPIV?2, and HPIV?3) are common respiratory paramyxoviruses. Here, we developed a two?tube triplex one?step real?time reverse?transcription polymerase chain reaction (real?time RT?PCR) and evaluated its performance using clinical samples. The data showed that this novel assay was 100% consistent with the monoplex real?time RT?PCR assay (in?house), which was superior to the commercial routine multiplex?ligation?NAT?based assay. Meanwhile, the clinical nasopharyngeal swabs of 471 patients with the acute febrile respiratory syndrome (AFRS) were analyzed using the established method. The results showed that 52 (11.7%) cases were positive for paramyxovirus. Among them, HPIVs and RSV?A had the highest detection rate. The age and seasonal distribution of human paramyxovirus infection were analyzed. In conclusion, we developed a novel multiplex real?time RT?PCR assay for the rapid detection of six common human paramyxoviruses, which were dominant in patients with AFRS in Qinghai. äDetection of six common human paramyxoviruses in patients with acute f(epmc).pdf DeepDyve Pubget Overpricing