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.jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117 J+Med+Virol
2019 ; 91
(6
): 963-971
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Th17 serum cytokines in relation to laboratory-confirmed respiratory viral
infection: A pilot study
#MMPMID30715745
Antalis E
; Spathis A
; Kottaridi C
; Kossyvakis A
; Pastellas K
; Tsakalos K
; Mentis A
; Kroupis C
; Tsiodras S
J Med Virol
2019[Jun]; 91
(6
): 963-971
PMID30715745
show ga
BACKGROUND: Th17 cytokines are associated with modulation of inflammation and may
be beneficial in clearing influenza infection in experimental models. The Th17
cytokine profile was evaluated in a pilot study of respiratory virus infections.
METHODS: Consecutive patients with symptoms of respiratory tract infection
visiting the emergency department of a tertiary care hospital during the winter
influenza season of 2014 to 2015 were evaluated. CLART PneumoVir kit, (GENOMICA,
Madrid, Spain) was used for viral detection of all known respiratory viruses.
Th17 cytokine profile was evaluated with the MILLIPLEX MAP Human TH17 Magnetic
Bead Panel (Millipore Corp., Billerica, MA). Correlation of the TH17 profile with
viral detection was performed with univariate and multivariate analysis. RESULTS:
Seventy-six patients were evaluated (median age 56 years, 51.3% female); a
respiratory virus was identified in 60 (78.9%) patients; 45% had confirmed
influenza. Influenza A (H3N2) correlated with higher levels of
granulocyte-macrophage colony-stimulating factor (GM-CSF), interleukin 1?
(IL-1?), IL-17A, IL-17E, IL-17F, IL-21, IL-22, and IL-23 (P?0.05 by analysis of
variance [ANOVA]) compared with respiratory syncytial virus (RSV). Parainfluenza
virus (PIV) similarly had higher levels of GM-CSF, IL-1b, IL-17A, IL-22 compared
with those detected in RSV, influenza B and any other virus infection ( P?0.05;
ANOVA). Increasing age (?-coefficient?=?1.11, 95% CI, 1.04-1.2, P?0.01) as well
as IL-17A levels (?-coefficient?=?1.03, 95% CI, 1.001-1.05, P?=?0.04) predicted
hospital admission. CONCLUSION: Main Th17 cell effector cytokines were
upregulated in laboratory-confirmed A(H3N2) influenza and PIV. Excessive amounts
of Th17 cytokines may be implicated in the pathogenesis and immune control of
acute influenza and PIV infection in humans and may predict the severity of
disease.