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10.1111/j.1365-2141.2012.09116.x http://scihub22266oqcxt.onion/10.1111/j.1365-2141.2012.09116.x C7161782!7161782!22489537     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Br+J+Haematol 2012 ; 157 (6): 709-17 Nephropedia Template TP {{tp|p=22489537|t=2012. Search for an aetiological virus candidate in chronic lymphocytic leukaemia by extensive transcriptome analysis |pdf=|usr=}}{{22489537}} gab.com Text Search for an aetiological virus candidate in chronic lymphocytic leukaemia by extensive transcriptome analysis JO: Br J Haematol http://www.kidney.de/pget.php?&tw=1&pmid=22489537 #FOAvip As an approach to determining the aetiology of chronic lymphocytic leukaemia (CLL), we searched for a virus expressed in human CLL B?cells by combining high?throughput sequencing and digital subtraction. Pooled B?cell mRNA transcriptomes from five CLL patients and five healthy donors were sequenced with 454 Life Sciences technology. Human reads were excluded by BLAST (Basic Local Alignment Search Tool) and BLAT (BLAST?like alignment tool) searches. Remaining reads were screened with BLAST against viral databases. Purified B?cells from two CLL patients, with and without stimulation by phorbol?esters, were sequenced using Illumina technology to achieve depth of sequencing. Burrows?Wheeler Aligner mapping and BLAST searches were used for the Illumina data. Pyrosequencing resulted in about 400 000 reads per sample. No viral candidate could be found. Illumina single?end sequencing for 115 cycles yielded an average of 26 ± 2·5 million filtered reads per sample, of which 2·2 ± 0·6 million remained unmapped to human references. BLAST searches of these reads against viral and human databases assigned nine reads to an Epstein?Barr virus origin, in one sample following phorbol?ester stimulation. Other reads showing a putative viral origin were dismissed after further analysis. Despite an in?depth analysis of the CLL transcriptome reaching more than 100 million sequences, we have not found evidence for a putative viral candidate in CLL. Twit Text FOAVip Search for an aetiological virus candidate in chronic lymphocytic leukaemia by extensive transcriptome analysis ????Br J Haematol http://www.kidney.de/pget.php?&tw=1&pmid=22489537 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=22489537 #FOAvip English Wikipedia <ref>{{Cite pmid|22489537}}</ref> Search for an aetiological virus candidate in chronic lymphocytic leukaemia by extensive transcriptome analysis #MMPMID22489537 Rego N; Bianchi S; Moreno P; Persson H; Kvist A; Pena A; Oppezzo P; Naya H; Rovira C; Dighiero G; Pritsch O Br J Haematol 2012[Jun]; 157 (6): 709-17 PMID22489537show ga As an approach to determining the aetiology of chronic lymphocytic leukaemia (CLL), we searched for a virus expressed in human CLL B?cells by combining high?throughput sequencing and digital subtraction. Pooled B?cell mRNA transcriptomes from five CLL patients and five healthy donors were sequenced with 454 Life Sciences technology. Human reads were excluded by BLAST (Basic Local Alignment Search Tool) and BLAT (BLAST?like alignment tool) searches. Remaining reads were screened with BLAST against viral databases. Purified B?cells from two CLL patients, with and without stimulation by phorbol?esters, were sequenced using Illumina technology to achieve depth of sequencing. Burrows?Wheeler Aligner mapping and BLAST searches were used for the Illumina data. Pyrosequencing resulted in about 400 000 reads per sample. No viral candidate could be found. Illumina single?end sequencing for 115 cycles yielded an average of 26 ± 2·5 million filtered reads per sample, of which 2·2 ± 0·6 million remained unmapped to human references. BLAST searches of these reads against viral and human databases assigned nine reads to an Epstein?Barr virus origin, in one sample following phorbol?ester stimulation. Other reads showing a putative viral origin were dismissed after further analysis. Despite an in?depth analysis of the CLL transcriptome reaching more than 100 million sequences, we have not found evidence for a putative viral candidate in CLL. äSearch for an aetiological virus candidate in chronic lymphocytic leuk(epmc).pdf DeepDyve Pubget Overpricing