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10.1016/j.mcp.2020.101570 http://scihub22266oqcxt.onion/10.1016/j.mcp.2020.101570 C7158812!7158812!32304824     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Mol+Cell+Probes 2020 ; 52 (ä): 101570 Nephropedia Template TP {{tp|p=32304824|t=2020. Development of qPCR platform with probes for quantifying prevalent and biomedically relevant human gut microbial taxa |pdf=|usr=}}{{32304824}} gab.com Text Development of qPCR platform with probes for quantifying prevalent and biomedically relevant human gut microbial taxa JO: Mol Cell Probes http://www.kidney.de/pget.php?&tw=1&pmid=32304824 #FOAvip Nowadays the advent of innovative high-throughput sequencing allows obtaining high-quality microbiome profiling. However, PCR-based tests are still considered the ?golden standard? for many clinical applications. Here, we designed a qPCR-based platform with fluorescent-labeled oligonucleotide probes for assessing human gut microbiome composition. The system allows conducting qualitative and semiquantitative analysis for 12 prokaryotic taxa that are prevalent in the human gut and associated with diseases, diet, age and other factors. The platform was validated by comparing microbiome profile data obtained with two different methods - the platform and high-throughput 16S rRNA sequencing - across 42 stool samples. The test can form the basis for precise and cost-efficient microbiome assay for large-scale surveys including clinical trials with interventions related to diet and disease risks. Twit Text FOAVip Development of qPCR platform with probes for quantifying prevalent and biomedically relevant human gut microbial taxa ????Mol Cell Probes http://www.kidney.de/pget.php?&tw=1&pmid=32304824 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=32304824 #FOAvip English Wikipedia <ref>{{Cite pmid|32304824}}</ref> Development of qPCR platform with probes for quantifying prevalent and biomedically relevant human gut microbial taxa #MMPMID32304824 Kurina I; Popenko A; Klimenko N; Koshechkin S; Chuprikova L; Filipenko M; Tyakht A; Alexeev D Mol Cell Probes 2020[Aug]; 52 (ä): 101570 PMID32304824show ga Nowadays the advent of innovative high-throughput sequencing allows obtaining high-quality microbiome profiling. However, PCR-based tests are still considered the ?golden standard? for many clinical applications. Here, we designed a qPCR-based platform with fluorescent-labeled oligonucleotide probes for assessing human gut microbiome composition. The system allows conducting qualitative and semiquantitative analysis for 12 prokaryotic taxa that are prevalent in the human gut and associated with diseases, diet, age and other factors. The platform was validated by comparing microbiome profile data obtained with two different methods - the platform and high-throughput 16S rRNA sequencing - across 42 stool samples. The test can form the basis for precise and cost-efficient microbiome assay for large-scale surveys including clinical trials with interventions related to diet and disease risks. äDevelopment of qPCR platform with probes for quantifying prevalent and(epmc).pdf DeepDyve Pubget Overpricing