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10.1016/j.virol.2005.06.016

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suck abstract from ncbi


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pmid16043204
      Virology 2005 ; 340 (2 ): 174-82
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  • Long-term protection from SARS coronavirus infection conferred by a single immunization with an attenuated VSV-based vaccine #MMPMID16043204
  • Kapadia SU ; Rose JK ; Lamirande E ; Vogel L ; Subbarao K ; Roberts A
  • Virology 2005[Sep]; 340 (2 ): 174-82 PMID16043204 show ga
  • Although the recent SARS coronavirus (SARS-CoV) that appeared in 2002 has now been contained, the possibility of re-emergence of SARS-CoV remains. Due to the threat of re-emergence, the overall fatality rate of approximately 10%, and the rapid dispersion of the virus via international travel, viable vaccine candidates providing protection from SARS are clearly needed. We developed an attenuated VSV recombinant (VSV-S) expressing the SARS coronavirus (SARS-CoV) spike (S) protein. In cells infected with this recombinant, S protein was synthesized, glycosylated at approximately 17 Asn residues, and transported via the Golgi to the cell surface. Mice vaccinated with VSV-S developed SARS-neutralizing antibody and were able to control a challenge with SARS-CoV performed at 1 month or 4 months after a single vaccination. We also demonstrated, by passive antibody transfer, that the antibody response induced by the vaccine was sufficient for controlling SARS-CoV infection. A VSV-vectored SARS vaccine could have significant advantages over other SARS vaccine candidates described to date.
  • |Animals [MESH]
  • |Antibody Formation [MESH]
  • |Cell Line [MESH]
  • |Cricetinae [MESH]
  • |DNA Primers [MESH]
  • |Immunity, Cellular [MESH]
  • |Kidney [MESH]
  • |Mice [MESH]
  • |Reverse Transcriptase Polymerase Chain Reaction [MESH]
  • |Severe Acute Respiratory Syndrome/*immunology [MESH]
  • |Severe acute respiratory syndrome-related coronavirus/genetics/*immunology [MESH]
  • |Vaccines, Attenuated [MESH]
  • |Vaccines, DNA [MESH]
  • |Vesicular stomatitis Indiana virus/*immunology [MESH]


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