Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=31493768
&cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests
in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215
Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\31493768
.jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117 Pediatr+Res
2020 ; 87
(3
): 463-471
Nephropedia Template TP
gab.com Text
Twit Text FOAVip
Twit Text #
English Wikipedia
Magnesium sulfate inhibits inflammation through P2X7 receptors in human umbilical
vein endothelial cells
#MMPMID31493768
Ozen M
; Xie H
; Shin N
; Al Yousif G
; Clemens J
; McLane MW
; Lei J
; Burd I
Pediatr Res
2020[Feb]; 87
(3
): 463-471
PMID31493768
show ga
BACKGROUND: Magnesium sulfate (MgSO(4)) is utilized for fetal neuroprotection in
preterm birth but its mechanism of action is still poorly understood. P2X7
receptor (P2X7R) is required for secretion of IL-1?, and can be blocked by
divalent cations such as magnesium (Mg) and its own antagonist, Brilliant Blue G
(BBG). We sought to determine whether during inflammation MgSO(4) can block
endothelial IL-1? secretion, using an in-vitro model. METHODS: Human umbilical
vein endothelial cell (HUVEC) cultures were treated with varying doses of LPS,
2'(3)-?-(4-Benzoylbenzoyl) adenosine-5'-triphosphate (BzATP), BBG and MgSO(4) for
3- or 24?h. We determined cell cytotoxicity, apoptosis, IL-1? mRNA expression,
IL-1? production and secretion and P2X7R expression on HUVECs. RESULTS: We
demonstrated that MgSO(4) is efficacious in blocking IL-1?-mediated-inflammation
in HUVECs, at both the initiation and propagation phases of inflammation. MgSO(4)
exerts these anti-inflammatory effects via downregulation of P2X7Rs on HUVECs.
CONCLUSION: LPS-exposure increases IL-1? production and secretion in HUVECs,
which is further intensified by P2X7R agonist, BzATP while MgSO(4) inhibits IL-1?
in both presence and absence of BzATP. This effect is similar to the results of
P2X7R antagonist, BBG, suggesting that the anti-inflammatory effects of MgSO(4)
is through P2X7R.
free
free
free
