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10.1038/s41467-019-09733-6

http://scihub22266oqcxt.onion/10.1038/s41467-019-09733-6
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suck abstract from ncbi


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pmid30979900      Nat+Commun 2019 ; 10 (ä): ä
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  • Immunoregulation of macrophages by dynamic ligand presentation via ligand?cation coordination #MMPMID30979900
  • Kang H; Yang B; Zhang K; Pan Q; Yuan W; Li G; Bian L
  • Nat Commun 2019[]; 10 (ä): ä PMID30979900show ga
  • Macrophages regulate host responses to implants through their dynamic adhesion, release, and activation. Herein, we employ bisphosphonate (BP)-coated gold nanoparticle template (BNP) to direct the swift and convertible formation of Mg2+-functional Mg2+-BP nanoparticle (NP) on the BP-AuNP surface via reversible Mg2+-BP coordination, thus producing (Mg2+-BP)-Au dimer (MgBNP). Ethylenediaminetetraacetic acid-based Mg2+ chelation facilitates the dissolution of Mg2+-BP NP, thus enabling the reversion of the MgBNP to the BNP. This convertible nanoassembly incorporating cell-adhesive Mg2+ moieties directs reversible attachment and detachment of macrophages by BP and EDTA, without physical scraping or trypsin that could damage cells. The swift formation of RGD ligand- and Mg2+-bifunctional RGD-Mg2+-BP NP that yields (RGD-Mg2+-BP)-Au dimer (RGDBNP) further stimulates the adhesion and pro-regenerative M2-type polarization of macrophages, both in vitro and in vivo, including rho-associated protein kinase. This swift and non-toxic dimer formation can include diverse bio-functional moieties to regulate host responses to implants.
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