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10.1038/s41467-018-05092-w http://scihub22266oqcxt.onion/10.1038/s41467-018-05092-w C6060171!6060171!30046034     free     free     free Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Nat+Commun 2018 ; 9 (ä): ä Nephropedia Template TP {{tp|p=30046034|t=2018. Widespread anti-CRISPR proteins in virulent bacteriophages inhibit a range of Cas9 proteins |pdf=|usr=}}{{30046034}} gab.com Text Widespread anti-CRISPR proteins in virulent bacteriophages inhibit a range of Cas9 proteins JO: Nat Commun http://www.kidney.de/pget.php?&tw=1&pmid=30046034 #FOAvip CRISPR-Cas systems are bacterial anti-viral systems, and bacterial viruses (bacteriophages, phages) can carry anti-CRISPR (Acr) proteins to evade that immunity. Acrs can also fine-tune the activity of CRISPR-based genome-editing tools. While Acrs are prevalent in phages capable of lying dormant in a CRISPR-carrying host, their orthologs have been observed only infrequently in virulent phages. Here we identify AcrIIA6, an Acr encoded in 33% of virulent Streptococcus thermophilus phage genomes. The X-ray structure of AcrIIA6 displays some features unique to this Acr family. We compare the activity of AcrIIA6 to those of other Acrs, including AcrIIA5 (also from S. thermophilus phages), and characterize their effectiveness against a range of CRISPR-Cas systems. Finally, we demonstrate that both Acr families from S. thermophilus phages inhibit Cas9-mediated genome editing of human cells. Twit Text FOAVip Widespread anti-CRISPR proteins in virulent bacteriophages inhibit a range of Cas9 proteins ????Nat Commun http://www.kidney.de/pget.php?&tw=1&pmid=30046034 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=30046034 #FOAvip English Wikipedia <ref>{{Cite pmid|30046034}}</ref> Widespread anti-CRISPR proteins in virulent bacteriophages inhibit a range of Cas9 proteins #MMPMID30046034 Hynes AP; Rousseau GM; Agudelo D; Goulet A; Amigues B; Loehr J; Romero DA; Fremaux C; Horvath P; Doyon Y; Cambillau C; Moineau S Nat Commun 2018[]; 9 (ä): ä PMID30046034show ga CRISPR-Cas systems are bacterial anti-viral systems, and bacterial viruses (bacteriophages, phages) can carry anti-CRISPR (Acr) proteins to evade that immunity. Acrs can also fine-tune the activity of CRISPR-based genome-editing tools. While Acrs are prevalent in phages capable of lying dormant in a CRISPR-carrying host, their orthologs have been observed only infrequently in virulent phages. Here we identify AcrIIA6, an Acr encoded in 33% of virulent Streptococcus thermophilus phage genomes. The X-ray structure of AcrIIA6 displays some features unique to this Acr family. We compare the activity of AcrIIA6 to those of other Acrs, including AcrIIA5 (also from S. thermophilus phages), and characterize their effectiveness against a range of CRISPR-Cas systems. Finally, we demonstrate that both Acr families from S. thermophilus phages inhibit Cas9-mediated genome editing of human cells. äWidespread anti-CRISPR proteins in virulent bacteriophages inhibit a r(epmc).pdf DeepDyve Pubget Overpricing