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2018 ; 40
(1
): 39-48
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MEG3/miR?21 axis affects cell mobility by suppressing epithelial?mesenchymal
transition in gastric cancer
#MMPMID29749532
Xu G
; Meng L
; Yuan D
; Li K
; Zhang Y
; Dang C
; Zhu K
Oncol Rep
2018[Jul]; 40
(1
): 39-48
PMID29749532
show ga
The prognosis of patients with gastric cancer remains poor mainly due to distant
metastasis. Maternally expressed gene 3 (MEG3), a long non?coding RNA (lncRNA),
is downregulated in various tumor tissues and suppresses tumor progression.
miR?21 is a microRNA which is expressed highly in tumor tissues. In the present
study, we investigated the relationship between MEG3 and miR?21 in regards to the
cell mobility of gastric cancer. Our data demonstrated that MEG3 was
downregulated while miR?21 was upregulated in gastric cancer tissues and cell
lines by qRT?PCR. Overexpression of MEG3 suppressed cell mobility of gastric
cancer cells (AGS) by downregulating the expression of MMP?3, MMP?9 and VEGF. As
shown by western blot analysis, overexpression of MEG3 also suppressed
epithelial?mesenchymal transition (EMT) by increasing the expression of an
epithelial marker (E?cadherin) and downregulating the expression of mesenchymal
markers (N?cadherin, Snail and ??catenin), indicating that MEG3 suppressed cell
mobility through the inhibition of EMT in gastric cancer. The expression of
miR?21 was negatively regulated by MEG3 and overexpression of miR?21 promoted
cell mobility of AGS through activation of EMT. Co?transfection of lncRNA?MEG3
and miR?21 mimic counteracted the inhibitory effect on the cell mobility
attributed to MEG3, suggesting that the MEG3/miR?21 axis affects cell mobility by
suppressing EMT in gastric cancer. Using a mouse xenograft tumor model, we found
that the overexpression of MEG3 suppressed tumor growth and metastasis while
overexpression of miR?21 had the opposite effects. The MEG3/miR?21 axis affected
gastric cancer growth and metastasis through inhibition of EMT in vivo. In
conclusion, we demonstrated that the MEG3/miR?21 axis participates in the tumor
progression and metastasis of gastric cancer through the regulation of EMT.
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