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.jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117 Oncol+Rep
2018 ; 40
(1
): 252-260
Nephropedia Template TP
gab.com Text
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MicroRNA-150 inhibits the proliferation and metastasis potential of colorectal
cancer cells by targeting iASPP
#MMPMID29750311
Li C
; Du X
; Xia S
; Chen L
Oncol Rep
2018[Jul]; 40
(1
): 252-260
PMID29750311
show ga
In the present study, the function of miR-150 and its downstream target iASPP in
the growth and metastasis of colorectal cancer (CRC) cells was investigated. The
expression of miR-150 and iASPP was first investigated in clinical CRC samples.
Subsequently, the effects of miR-150 overexpression and iASPP inhibition on cell
viability, cell cycle distribution, apoptosis, migration and invasion were
detected with CCK-8, flow cytometry, scratch and Transwell assays. The
interaction between miR-150 and iASPP was confirmed using a dual-luciferase
assay. Subsequently, the key role of iASPP in the anti-CRC function of miR-150
was assessed by inducing the expression of the gene in miR-150 overexpressed
SW480 cells. In clinical samples, the level of miR-150 was downregulated, while
iASPP was induced. Enforced expression of miR-150 decreased the viability,
induced G1 cell cycle arrest and apoptosis, and inhibited the migration and
invasion of SW480 cells. Knockdown of iASPP exerted a similar effect on SW480
cells to that of the overexpression of miR-150. Dual-luciferase assay
demonstrated that miR-150 directly bound to iASPP and inhibited its
transcription. The function of miR-150 depended on the inhibition of iASPP;
induced expression of iASPP in miR-150-knockdown SW480 and HCT116 cells restored
cell viability, migration and invasion while inhibiting G1 cell cycle arrest and
apoptosis. Increased expression of miR-150 suppressed viability, proliferation,
migration and invasion of SW480 cells. Furthermore, iASPP was a direct target of
miR-150 and played a key role in its anti-CRC function. miR-150 may be a
promising predictor of prognosis in CRC patients.
|Apoptosis
[MESH]
|Biomarkers, Tumor/*genetics
[MESH]
|Cell Cycle Checkpoints/genetics
[MESH]
|Cell Movement/genetics
[MESH]
|Cell Proliferation/genetics
[MESH]
|Cell Survival/genetics
[MESH]
|Colorectal Neoplasms/*genetics/pathology
[MESH]
|Gene Expression Regulation, Neoplastic
[MESH]
|Gene Knockdown Techniques
[MESH]
|HCT116 Cells
[MESH]
|Humans
[MESH]
|Intracellular Signaling Peptides and Proteins/antagonists & inhibitors/*genetics
[MESH]
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