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10.1038/s41598-018-29249-1 http://scihub22266oqcxt.onion/10.1038/s41598-018-29249-1 C6057954!6057954!30042404     free     free     free Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Sci+Rep 2018 ; 8 (ä): ä Nephropedia Template TP {{tp|p=30042404|t=2018. Quad-mode functional and molecular photoacoustic microscopy |pdf=|usr=}}{{30042404}} gab.com Text Quad-mode functional and molecular photoacoustic microscopy JO: Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=30042404 #FOAvip A conventional photoacoustic microscopy (PAM) system typically has to make tradeoffs between its spatial resolution and penetration depth, by choosing a fixed configuration of optical excitation and acoustic detection. The single-scale imaging capability of PAM may limit its applications in biomedical studies. Here, we report a quad-mode photoacoustic microscopy (QM-PAM) system with four complementary spatial resolutions and maximum penetration depths. For this we first developed a ring-shaped focused ultrasound transducer that has two independent elements with respective central frequencies at 20?MHz and 40?MHz, providing complementary acoustically-determined spatial resolutions and penetration depths. To accommodate the dual-element ultrasound transducer, we implemented two optical excitation modes to provide tightly- and weakly-focused light illumination. The dual-element acoustic detection combined with the two optical focusing modes can thus provide four imaging scales in a single imaging device, with consistent contrast mechanisms and co-registered field of views. We have demonstrated the multiscale morphological, functional, and molecular imaging capability of QM-PAM in the mouse head, leg and ear in vivo. We expect the high scale flexibility of QM-PAM will enable broad applications in preclinical studies. Twit Text FOAVip Quad-mode functional and molecular photoacoustic microscopy ????Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=30042404 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=30042404 #FOAvip English Wikipedia <ref>{{Cite pmid|30042404}}</ref> Quad-mode functional and molecular photoacoustic microscopy #MMPMID30042404 Liu W; Shcherbakova DM; Kurupassery N; Li Y; Zhou Q; Verkhusha VV; Yao J Sci Rep 2018[]; 8 (ä): ä PMID30042404show ga A conventional photoacoustic microscopy (PAM) system typically has to make tradeoffs between its spatial resolution and penetration depth, by choosing a fixed configuration of optical excitation and acoustic detection. The single-scale imaging capability of PAM may limit its applications in biomedical studies. Here, we report a quad-mode photoacoustic microscopy (QM-PAM) system with four complementary spatial resolutions and maximum penetration depths. For this we first developed a ring-shaped focused ultrasound transducer that has two independent elements with respective central frequencies at 20?MHz and 40?MHz, providing complementary acoustically-determined spatial resolutions and penetration depths. To accommodate the dual-element ultrasound transducer, we implemented two optical excitation modes to provide tightly- and weakly-focused light illumination. The dual-element acoustic detection combined with the two optical focusing modes can thus provide four imaging scales in a single imaging device, with consistent contrast mechanisms and co-registered field of views. We have demonstrated the multiscale morphological, functional, and molecular imaging capability of QM-PAM in the mouse head, leg and ear in vivo. We expect the high scale flexibility of QM-PAM will enable broad applications in preclinical studies. äQuad-mode functional and molecular photoacoustic microscopy (epmc).pdf DeepDyve Pubget Overpricing