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10.1038/s41467-018-05262-w http://scihub22266oqcxt.onion/10.1038/s41467-018-05262-w C6057936!6057936!30042426     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Nat+Commun 2018 ; 9 (ä): ä Nephropedia Template TP {{tp|p=30042426|t=2018. Introduction of pathogenic mutations into the mouse Psen1 gene by Base Editor and Target-AID |pdf=|usr=}}{{30042426}} gab.com Text Introduction of pathogenic mutations into the mouse Psen1 gene by Base Editor and Target-AID JO: Nat Commun http://www.kidney.de/pget.php?&tw=1&pmid=30042426 #FOAvip Base Editor (BE) and Target-AID (activation-induced cytidine deaminase) are engineered genome-editing proteins composed of Cas9 and cytidine deaminases. These base-editing tools convert C:G base pairs to T:A at target sites. Here, we inject either BE or Target-AID mRNA together with identical single-guide RNAs (sgRNAs) into mouse zygotes, and compare the base-editing efficiencies of the two distinct tools in vivo. BE consistently show higher base-editing efficiency (10.0?62.8%) compared to that of Target-AID (3.4?29.8%). However, unexpected base substitutions and insertion/deletion formations are also more frequently observed in BE-injected mice or zygotes. We are able to generate multiple mouse lines harboring point mutations in the mouse presenilin 1 (Psen1) gene by injection of BE or Target-AID. These results demonstrate that BE and Target-AID are highly useful tools to generate mice harboring pathogenic point mutations and to analyze the functional consequences of the mutations in vivo. Twit Text FOAVip Introduction of pathogenic mutations into the mouse Psen1 gene by Base Editor and Target-AID ????Nat Commun http://www.kidney.de/pget.php?&tw=1&pmid=30042426 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=30042426 #FOAvip English Wikipedia <ref>{{Cite pmid|30042426}}</ref> Introduction of pathogenic mutations into the mouse Psen1 gene by Base Editor and Target-AID #MMPMID30042426 Sasaguri H; Nagata K; Sekiguchi M; Fujioka R; Matsuba Y; Hashimoto S; Sato K; Kurup D; Yokota T; Saido TC Nat Commun 2018[]; 9 (ä): ä PMID30042426show ga Base Editor (BE) and Target-AID (activation-induced cytidine deaminase) are engineered genome-editing proteins composed of Cas9 and cytidine deaminases. These base-editing tools convert C:G base pairs to T:A at target sites. Here, we inject either BE or Target-AID mRNA together with identical single-guide RNAs (sgRNAs) into mouse zygotes, and compare the base-editing efficiencies of the two distinct tools in vivo. BE consistently show higher base-editing efficiency (10.0?62.8%) compared to that of Target-AID (3.4?29.8%). However, unexpected base substitutions and insertion/deletion formations are also more frequently observed in BE-injected mice or zygotes. We are able to generate multiple mouse lines harboring point mutations in the mouse presenilin 1 (Psen1) gene by injection of BE or Target-AID. These results demonstrate that BE and Target-AID are highly useful tools to generate mice harboring pathogenic point mutations and to analyze the functional consequences of the mutations in vivo. äIntroduction of pathogenic mutations into the mouse Psen1 gene by Base(epmc).pdf DeepDyve Pubget Overpricing