Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=28934848
&cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests
in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215
Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\28934848
.jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117 Cancer+Res+Treat
2018 ; 50
(3
): 883-893
Nephropedia Template TP
gab.com Text
Twit Text FOAVip
Twit Text #
English Wikipedia
Profiling of Serum Metabolites Using MALDI-TOF and Triple-TOF Mass Spectrometry
to Develop a Screen for Ovarian Cancer
#MMPMID28934848
Lee JH
; Kim YH
; Kim KH
; Cho JY
; Woo SM
; Yoo BC
; Kim SC
Cancer Res Treat
2018[Jul]; 50
(3
): 883-893
PMID28934848
show ga
PURPOSE: We sought to develop a matrix assisted laser desorption ionization-time
of flight (MALDI-TOF)-based, ovarian cancer (OVC), low-mass-ion discriminant
equation (LOME) and to evaluate a possible supportive role for triple-TOF mass
analysis in identifying metabolic biomarkers. MATERIALS AND METHODS: A total of
114 serum samples from patients with OVC and benign ovarian tumors were subjected
to MALDI-TOF analysis and a total of 137 serum samples from healthy female
individuals and patients with OVC, colorectal cancer, hepatobiliary cancer, and
pancreatic cancer were subjected to triple-TOF analysis. An OVC LOME was
constructed by reference to the peak intensity ratios of discriminatory low-mass
ion (LMI) pairs. Triple-TOF analysiswas used to select and identify metabolic
biomarkers for OVC screening. RESULTS: Three OVC LOMEs were finally constructed
using discriminatory LMI pairs (137.1690 and 84.4119 m/z; 496.5022 and 709.7642
m/z; and 524.5614 and 709.7642 m/z); all afforded accuracies of > 90%. The
LMIs at 496.5022 m/z and 524.5614 m/z were those of lysophosphatidylcholine (LPC)
16:0 and LPC 18:0. Triple-TOF analysis selected seven discriminative LMIs; each
LMI had a specificity > 90%. Of the seven LMIs, fourwith a 137.0455 m/z ion
atretention times of 2.04-3.14 minuteswere upregulated in sera from OVC patients;
the ion was identified as that derived from hypoxanthine. CONCLUSION:
MALDI-TOF-based OVC LOMEs combined with triple-TOF-based OVC metabolic biomarkers
allow reliable OVC screening; the techniques are mutually complementary both
quantitatively and qualitatively.
free
free
free
