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.jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117 Sci+Rep
2018 ; 8
(1
): 11023
Nephropedia Template TP
gab.com Text
Twit Text FOAVip
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English Wikipedia
The Effect of SkitoSnack, an Artificial Blood Meal Replacement, on Aedes aegypti
Life History Traits and Gut Microbiota
#MMPMID30038361
Gonzales KK
; Rodriguez SD
; Chung HN
; Kowalski M
; Vulcan J
; Moore EL
; Li Y
; Willette SM
; Kandel Y
; Van Voorhies WA
; Holguin FO
; Hanley KA
; Hansen IA
Sci Rep
2018[Jul]; 8
(1
): 11023
PMID30038361
show ga
Public health research and vector control frequently require the rearing of large
numbers of vector mosquitoes. All target vector mosquito species are
anautogenous, meaning that females require vertebrate blood for egg production.
Vertebrate blood, however, is costly, with a short shelf life. To overcome these
constraints, we have developed SkitoSnack, an artificial blood meal replacement
for the mosquito Aedes aegypti, the vector of dengue, Zika and chikungunya virus.
SkitoSnack contains bovine serum albumin and hemoglobin as protein source as well
as egg yolk and a bicarbonate buffer. SkitoSnack-raised females had comparable
life history traits as blood-raised females. Mosquitoes reared from
SkitoSnack-fed females had similar levels of infection and dissemination when
orally challenged with dengue virus type 2 (DENV-2) and significantly lower
infection with DENV-4. When SkitoSnack was used as a vehicle for DENV-2 delivery,
blood-raised and SkitoSnack-raised females were equally susceptible. The midgut
microbiota differed significantly between mosquitoes fed on SkitoSnack and
mosquitoes fed on blood. By rearing 20 generations of Aedes exclusively on
SkitoSnack, we have proven that this artificial diet can replace blood in
mosquito mass rearing.