Use my Search Websuite to scan PubMed, PMCentral, Journal Hosts and Journal Archives, FullText. Kick-your-searchterm to multiple Engines kick-your-query now !>

A dictionary by aggregated review articles of nephrology, medicine and the life sciences Your one-stop-run pathway from word to the immediate pdf of peer-reviewed on-topic knowledge.

10.14814/phy2.13796 http://scihub22266oqcxt.onion/10.14814/phy2.13796 C6055029!6055029 !30033625     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=30033625 &cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 251.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 251.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 251.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 251.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 251.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\30033625 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Physiol+Rep 2018 ; 6 (14 ): e13796 Nephropedia Template TP {{tp|p=30033625 |t=2018. TRPM7-mediated spontaneous Ca(2+) entry regulates the proliferation and differentiation of human leukemia cell line K562 |pdf=|usr=}}{{30033625 }} gab.com Text TRPM7-mediated spontaneous Ca(2+) entry regulates the proliferation and differentiation of human leukemia cell line K562 JO: Physiol Rep http://www.kidney.de/pget.php?&tw=1&pmid=30033625 #FOAvip Continuous Ca(2+) influx is essential to maintain intracellular Ca(2+) homeostasis and its dysregulation leads to a variety of cellular dysfunctions. In this study, we explored the functional roles of spontaneous Ca(2+) influx for the proliferation and differentiation of a human erythromyeloid leukemia cell line K562. mRNA/protein expressions were assessed by the real-time RT-PCR, western blotting, and immunocytochemical staining. Intracellular Ca(2+) concentration ([Ca(2+) ](i) ) and ionic currents were measured by fluorescent imaging and patch clamping techniques, respectively. Cell counting/viability and colorimetric assays were applied to assess proliferation rate and hemoglobin synthesis, respectively. Elimination of extracellular Ca(2+) decreased basal [Ca(2+) ](i) in proliferating K562 cells. Cation channel blockers such as SK&F96365, 2-APB, Gd(3+) , and FTY720 dose dependently decreased basal [Ca(2+) ](i) . A spontaneously active inward current (I(spont) ) contributive to basal [Ca(2+) ](i) was identified by the nystatin-perforated whole-cell recording. I(spont) permeated Ca(2+) comparably to Na(+) , and was greatly eliminated by siRNA targeting TRPM7, a melastatin member of the transient receptor potential (TRP) superfamily. Consistent with these findings, TRPM7 immune reactivity was detected by western blotting, and immunofluorescence representing TRPM7 was found localized to the K562 cell membrane. Strikingly, all these procedures, that is, Ca(2+) removal, TRPM7 blockers and siRNA-mediated TRPM7 knockdown significantly retarded the growth and suppressed hemin-induced ?-globin and hemoglobin syntheses in K562 cells, respectively, both of which appeared associated with the inhibition of ERK activation. These results collectively suggest that spontaneous Ca(2+) influx through constitutively active TRPM7 channels may critically regulate both proliferative and erythroid differentiation potentials of K562 cells. Twit Text FOAVip TRPM7-mediated spontaneous Ca(2+) entry regulates the proliferation and differentiation of human leukemia cell line K562 ????Physiol Rep http://www.kidney.de/pget.php?&tw=1&pmid=30033625 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=30033625 #FOAvip English Wikipedia <ref>{{Cite pmid|30033625 }}</ref> TRPM7-mediated spontaneous Ca(2+) entry regulates the proliferation and differentiation of human leukemia cell line K562 #MMPMID30033625 Takahashi K ; Umebayashi C ; Numata T ; Honda A ; Ichikawa J ; Hu Y ; Yamaura K ; Inoue R Physiol Rep 2018[Jul]; 6 (14 ): e13796 PMID30033625 show ga Continuous Ca(2+) influx is essential to maintain intracellular Ca(2+) homeostasis and its dysregulation leads to a variety of cellular dysfunctions. In this study, we explored the functional roles of spontaneous Ca(2+) influx for the proliferation and differentiation of a human erythromyeloid leukemia cell line K562. mRNA/protein expressions were assessed by the real-time RT-PCR, western blotting, and immunocytochemical staining. Intracellular Ca(2+) concentration ([Ca(2+) ](i) ) and ionic currents were measured by fluorescent imaging and patch clamping techniques, respectively. Cell counting/viability and colorimetric assays were applied to assess proliferation rate and hemoglobin synthesis, respectively. Elimination of extracellular Ca(2+) decreased basal [Ca(2+) ](i) in proliferating K562 cells. Cation channel blockers such as SK&F96365, 2-APB, Gd(3+) , and FTY720 dose dependently decreased basal [Ca(2+) ](i) . A spontaneously active inward current (I(spont) ) contributive to basal [Ca(2+) ](i) was identified by the nystatin-perforated whole-cell recording. I(spont) permeated Ca(2+) comparably to Na(+) , and was greatly eliminated by siRNA targeting TRPM7, a melastatin member of the transient receptor potential (TRP) superfamily. Consistent with these findings, TRPM7 immune reactivity was detected by western blotting, and immunofluorescence representing TRPM7 was found localized to the K562 cell membrane. Strikingly, all these procedures, that is, Ca(2+) removal, TRPM7 blockers and siRNA-mediated TRPM7 knockdown significantly retarded the growth and suppressed hemin-induced ?-globin and hemoglobin syntheses in K562 cells, respectively, both of which appeared associated with the inhibition of ERK activation. These results collectively suggest that spontaneous Ca(2+) influx through constitutively active TRPM7 channels may critically regulate both proliferative and erythroid differentiation potentials of K562 cells. |*Calcium Signaling [MESH] |*Cell Proliferation [MESH] |*Erythropoiesis [MESH] |Cell Line, Tumor [MESH] |Humans [MESH] |Leukemia/*metabolism [MESH] |Protein Serine-Threonine Kinases/genetics/*metabolism [MESH]TRPM7-mediated spontaneous Ca(2+) entry regulates the proliferation an(epmc).pdf DeepDyve Pubget Overpricing