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.jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117 PLoS+One
2018 ; 13
(7
): e0200945
Nephropedia Template TP
gab.com Text
Twit Text FOAVip
Twit Text #
English Wikipedia
Urine proteome analysis by C18 plate-matrix-assisted laser desorption/ionization
time-of-flight mass spectrometry allows noninvasive differential diagnosis and
prediction of diabetic nephropathy
#MMPMID30024955
Chen CJ
; Liao WL
; Chang CT
; Liao HY
; Tsai FJ
PLoS One
2018[]; 13
(7
): e0200945
PMID30024955
show ga
Diabetic nephropathy (DN) is one of the most common complications in diabetic
patients. New noninvasive markers are still needed for the early detection of DN
before identifiable alternations in kidney function or urine albumin excretion
occurs. A C18 plate and matrix-assisted laser desorption/ionization
time-of-flight mass spectrometry (MALDI-TOF-MS) were used to compare the urinary
protein profiles of 238 subjects from the following 4 groups: patients with type
2 diabetic (T2D) with microalbuminuria, patients with DM without micro- or
macroalbuminuria, patients with micro- or macroalbuminuria due to nondiabetic
disease, and healthy controls. ?2-microglobulin (B2M) and Clara-cell protein
(CC16) were found to be highly released in the urine of patients with proteinuria
due to nondiabetic or diabetic diseases. In differentiating nephropathy from
healthy subject, the B2M and CC16 markers have a combined sensitivity and
specificity of 77.3% and 91.8%, respectively. In distinguishing T2D with
microalbuminuria from T2D patients, the combined markers have sensitivity and
specificity of 66% and 73%, respectively. The predictive ability of B2M and CC16
for early renal functional decline (ERFD) was validated in 125 T2D patients with
a follow-up times. The odds ratio (OR) of combined B2M and CC16 markers for
developing ERFD was 7.59 (95% CI: 1.97-29.24). The detection of B2M and CC16 with
the C18 plate-MALDI-TOF MS approach could be an attractive and practical assay
for rapid diagnosis of nephropathy in nondiabetic/diabetic patients and as a
predictor of ERFD among T2D patients who had not manifested significant kidney
disease at baseline.