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10.1038/s41598-018-29123-0 http://scihub22266oqcxt.onion/10.1038/s41598-018-29123-0 C6052003!6052003 !30022089     free     free     free Deprecated: Implicit conversion from float 225.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 225.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 225.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 225.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 225.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 225.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 225.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 225.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 225.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\30022089 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Sci+Rep 2018 ; 8 (1 ): 10849 Nephropedia Template TP {{tp|p=30022089 |t=2018. Inactivation of TCA cycle enhances Staphylococcus aureus persister cell formation in stationary phase |pdf=|usr=}}{{30022089 }} gab.com Text Inactivation of TCA cycle enhances Staphylococcus aureus persister cell formation in stationary phase JO: Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=30022089 #FOAvip Persister cells constitute a small subpopulation of bacteria that display remarkably high antibiotic tolerance and for pathogens such as Staphylococcus aureus are suspected as culprits of chronic and recurrent infections. Persisters formed during exponential growth are characterized by low ATP levels but less is known of cells in stationary phase. By enrichment from a transposon mutant library in S. aureus we identified mutants that in this growth phase displayed enhanced persister cell formation. We found that inactivation of either sucA or sucB, encoding the subunits of the ?-ketoglutarate dehydrogenase of the tricarboxylic acid cycle (TCA cycle), increased survival to lethal concentrations of ciprofloxacin by 10-100 fold as did inactivation of other TCA cycle genes or atpA encoding a subunit of the F(1)F(0) ATPase. In S. aureus, TCA cycle activity and gene expression are de-repressed in stationary phase but single cells with low expression may be prone to form persisters. While ATP levels were not consistently affected in high persister mutants they commonly displayed reduced membrane potential, and persistence was enhanced by a protein motive force inhibitor. Our results show that persister cell formation in stationary phase does not correlate with ATP levels but is associated with low membrane potential. Twit Text FOAVip Inactivation of TCA cycle enhances Staphylococcus aureus persister cell formation in stationary phase ????Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=30022089 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=30022089 #FOAvip English Wikipedia <ref>{{Cite pmid|30022089 }}</ref> Inactivation of TCA cycle enhances Staphylococcus aureus persister cell formation in stationary phase #MMPMID30022089 Wang Y ; Bojer MS ; George SE ; Wang Z ; Jensen PR ; Wolz C ; Ingmer H Sci Rep 2018[Jul]; 8 (1 ): 10849 PMID30022089 show ga Persister cells constitute a small subpopulation of bacteria that display remarkably high antibiotic tolerance and for pathogens such as Staphylococcus aureus are suspected as culprits of chronic and recurrent infections. Persisters formed during exponential growth are characterized by low ATP levels but less is known of cells in stationary phase. By enrichment from a transposon mutant library in S. aureus we identified mutants that in this growth phase displayed enhanced persister cell formation. We found that inactivation of either sucA or sucB, encoding the subunits of the ?-ketoglutarate dehydrogenase of the tricarboxylic acid cycle (TCA cycle), increased survival to lethal concentrations of ciprofloxacin by 10-100 fold as did inactivation of other TCA cycle genes or atpA encoding a subunit of the F(1)F(0) ATPase. In S. aureus, TCA cycle activity and gene expression are de-repressed in stationary phase but single cells with low expression may be prone to form persisters. While ATP levels were not consistently affected in high persister mutants they commonly displayed reduced membrane potential, and persistence was enhanced by a protein motive force inhibitor. Our results show that persister cell formation in stationary phase does not correlate with ATP levels but is associated with low membrane potential. |*Citric Acid Cycle [MESH] |Adenosine Triphosphate/*metabolism [MESH] |Anti-Bacterial Agents/*pharmacology [MESH] |Drug Resistance, Multiple, Bacterial [MESH] |Gene Expression Regulation, Bacterial [MESH] |Ketoglutarate Dehydrogenase Complex/*antagonists & inhibitors [MESH] |Membrane Potentials [MESH] |Staphylococcal Infections/drug therapy/metabolism/*microbiology [MESH]Inactivation of TCA cycle enhances Staphylococcus aureus persister cel(epmc).pdf DeepDyve Pubget Overpricing