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2018 ; 5
(ä): 180129
Nephropedia Template TP
gab.com Text
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English Wikipedia
Leukocyte Tracking Database, a collection of immune cell tracks from intravital
2-photon microscopy videos
#MMPMID30015806
Pizzagalli DU
; Farsakoglu Y
; Palomino-Segura M
; Palladino E
; Sintes J
; Marangoni F
; Mempel TR
; Koh WH
; Murooka TT
; Thelen F
; Stein JV
; Pozzi G
; Thelen M
; Krause R
; Gonzalez SF
Sci Data
2018[Jul]; 5
(ä): 180129
PMID30015806
show ga
Recent advances in intravital video microscopy have allowed the visualization of
leukocyte behavior in vivo, revealing unprecedented spatiotemporal dynamics of
immune cell interaction. However, state-of-the-art software and methods for
automatically measuring cell migration exhibit limitations in tracking the
position of leukocytes over time. Challenges arise both from the complex
migration patterns of these cells and from the experimental artifacts introduced
during image acquisition. Additionally, the development of novel tracking tools
is hampered by the lack of a sound ground truth for algorithm validation and
benchmarking. Therefore, the objective of this work was to create a database,
namely LTDB, with a significant number of manually tracked leukocytes. Broad
experimental conditions, sites of imaging, types of immune cells and challenging
case studies were included to foster the development of robust computer vision
techniques for imaging-based immunological research. Lastly, LTDB represents a
step towards the unravelling of biological mechanisms by video data mining in
systems biology.