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10.1371/journal.pbio.2005840 http://scihub22266oqcxt.onion/10.1371/journal.pbio.2005840 C6049001!6049001!30011268     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       PLoS+Biol 2018 ; 16 (7): ä Nephropedia Template TP {{tp|p=30011268|t=2018. In vitro?transcribed guide RNAs trigger an innate immune response via the RIG-I pathway |pdf=|usr=}}{{30011268}} gab.com Text In vitro transcribed guide RNAs trigger an innate immune response via the RIG-I pathway JO: PLoS Biol http://www.kidney.de/pget.php?&tw=1&pmid=30011268 #FOAvip Clustered, regularly interspaced, short palindromic repeat (CRISPR)?CRISPR-associated 9 (Cas9) genome editing is revolutionizing fundamental research and has great potential for the treatment of many diseases. While editing of immortalized cell lines has become relatively easy, editing of therapeutically relevant primary cells and tissues can remain challenging. One recent advancement is the delivery of a Cas9 protein and an in vitro?transcribed (IVT) guide RNA (gRNA) as a precomplexed ribonucleoprotein (RNP). This approach allows editing of primary cells such as T cells and hematopoietic stem cells, but the consequences beyond genome editing of introducing foreign Cas9 RNPs into mammalian cells are not fully understood. Here, we show that the IVT gRNAs commonly used by many laboratories for RNP editing trigger a potent innate immune response that is similar to canonical immune-stimulating ligands. IVT gRNAs are recognized in the cytosol through the retinoic acid?inducible gene I (RIG-I) pathway but not the melanoma differentiation?associated gene 5 (MDA5) pathway, thereby triggering a type I interferon response. Removal of the 5?-triphosphate from gRNAs ameliorates inflammatory signaling and prevents the loss of viability associated with genome editing in hematopoietic stem cells. The potential for Cas9 RNP editing to induce a potent antiviral response indicates that care must be taken when designing therapeutic strategies to edit primary cells. Twit Text FOAVip In vitro transcribed guide RNAs trigger an innate immune response via the RIG-I pathway ????PLoS Biol http://www.kidney.de/pget.php?&tw=1&pmid=30011268 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=30011268 #FOAvip English Wikipedia <ref>{{Cite pmid|30011268}}</ref> In vitro?transcribed guide RNAs trigger an innate immune response via the RIG-I pathway #MMPMID30011268 Wienert B; Shin J; Zelin E; Pestal K; Corn JE PLoS Biol 2018[Jul]; 16 (7): ä PMID30011268show ga Clustered, regularly interspaced, short palindromic repeat (CRISPR)?CRISPR-associated 9 (Cas9) genome editing is revolutionizing fundamental research and has great potential for the treatment of many diseases. While editing of immortalized cell lines has become relatively easy, editing of therapeutically relevant primary cells and tissues can remain challenging. One recent advancement is the delivery of a Cas9 protein and an in vitro?transcribed (IVT) guide RNA (gRNA) as a precomplexed ribonucleoprotein (RNP). This approach allows editing of primary cells such as T cells and hematopoietic stem cells, but the consequences beyond genome editing of introducing foreign Cas9 RNPs into mammalian cells are not fully understood. Here, we show that the IVT gRNAs commonly used by many laboratories for RNP editing trigger a potent innate immune response that is similar to canonical immune-stimulating ligands. IVT gRNAs are recognized in the cytosol through the retinoic acid?inducible gene I (RIG-I) pathway but not the melanoma differentiation?associated gene 5 (MDA5) pathway, thereby triggering a type I interferon response. Removal of the 5?-triphosphate from gRNAs ameliorates inflammatory signaling and prevents the loss of viability associated with genome editing in hematopoietic stem cells. The potential for Cas9 RNP editing to induce a potent antiviral response indicates that care must be taken when designing therapeutic strategies to edit primary cells. äIn vitro?transcribed guide RNAs trigger an innate immune response via (epmc).pdf DeepDyve Pubget Overpricing