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10.1016/j.isci.2018.06.001 http://scihub22266oqcxt.onion/10.1016/j.isci.2018.06.001 C6048970!6048970!30027155     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       iScience 2018 ; 4 (ä): 216-35 Nephropedia Template TP {{tp|p=30027155|t=2018. Live-Cell Imaging of Chromatin Condensation Dynamics by CRISPR |pdf=|usr=}}{{30027155}} gab.com Text Live-Cell Imaging of Chromatin Condensation Dynamics by CRISPR JO: iScience http://www.kidney.de/pget.php?&tw=1&pmid=30027155 #FOAvip The spatiotemporal organization of chromatin plays central roles in cellular function. The ribosomal DNA (rDNA) chromatin undergoes dynamic structural changes during mitosis and stress. Here, we developed a CRISPR-based imaging system and tracked the condensation dynamics of rDNA chromatin in live yeast cells under glucose starvation. We found that acute glucose starvation triggers rapid condensation of rDNA. Time-lapse microscopy revealed two stages for rDNA condensation: a ?primary stage,? when relaxed rDNA chromatin forms higher order loops or rings, and a ?secondary stage,? when the rDNA rings further condense into compact clusters. Twisting of rDNA rings accompanies the secondary stage. The condensin complex, but not the cohesin complex, is required for efficient rDNA condensation in response to glucose starvation. Furthermore, we found that the DNA helicase Sgs1 is essential for the survival of cells expressing rDNA-bound dCas9, suggesting a role for helicases in facilitating DNA replication at dCas9-binding sites. Twit Text FOAVip Live-Cell Imaging of Chromatin Condensation Dynamics by CRISPR ????iScience http://www.kidney.de/pget.php?&tw=1&pmid=30027155 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=30027155 #FOAvip English Wikipedia <ref>{{Cite pmid|30027155}}</ref> Live-Cell Imaging of Chromatin Condensation Dynamics by CRISPR #MMPMID30027155 Xue Y; Acar M iScience 2018[Jun]; 4 (ä): 216-35 PMID30027155show ga The spatiotemporal organization of chromatin plays central roles in cellular function. The ribosomal DNA (rDNA) chromatin undergoes dynamic structural changes during mitosis and stress. Here, we developed a CRISPR-based imaging system and tracked the condensation dynamics of rDNA chromatin in live yeast cells under glucose starvation. We found that acute glucose starvation triggers rapid condensation of rDNA. Time-lapse microscopy revealed two stages for rDNA condensation: a ?primary stage,? when relaxed rDNA chromatin forms higher order loops or rings, and a ?secondary stage,? when the rDNA rings further condense into compact clusters. Twisting of rDNA rings accompanies the secondary stage. The condensin complex, but not the cohesin complex, is required for efficient rDNA condensation in response to glucose starvation. Furthermore, we found that the DNA helicase Sgs1 is essential for the survival of cells expressing rDNA-bound dCas9, suggesting a role for helicases in facilitating DNA replication at dCas9-binding sites. äLive-Cell Imaging of Chromatin Condensation Dynamics by CRISPR (epmc).pdf DeepDyve Pubget Overpricing