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10.1152/ajpheart.00512.2017

http://scihub22266oqcxt.onion/10.1152/ajpheart.00512.2017
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C6048465!6048465!29351459
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suck abstract from ncbi


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pmid29351459      Am+J+Physiol+Heart+Circ+Physiol 2018 ; 314 (4): H724-32
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  • Guidelines for authors and reviewers on antibody use in physiology studies #MMPMID29351459
  • Brooks HL; Lindsey ML
  • Am J Physiol Heart Circ Physiol 2018[Apr]; 314 (4): H724-32 PMID29351459show ga
  • Antibody use is a critical component of cardiovascular physiology research, and antibodies are used to monitor protein abundance (immunoblot analysis) and protein expression and localization (in tissue by immunohistochemistry and in cells by immunocytochemistry). With ongoing discussions on how to improve reproducibility and rigor, the goal of this review is to provide best practice guidelines regarding how to optimize antibody use for increased rigor and reproducibility in both immunoblot analysis and immunohistochemistry approaches.Listen to this article?s corresponding podcast at http://ajpheart.podbean.com/e/guidelines-on-antibody-use-in-physiology-studies/.
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