Triptolide inhibits TGF-?-induced matrix contraction and fibronectin production
mediated by human Tenon fibroblasts
#MMPMID30046525
Liu Y
; Liu PP
; Liu L
; Zheng XS
; Zheng H
; Yang CC
; Luobu CR
; Liu Y
Int J Ophthalmol
2018[]; 11
(7
): 1108-1113
PMID30046525
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AIM: To determine if triptolide influences the contractility and fibronectin
production in human Tenon fibroblasts (HTFs). METHODS: HTFs were cultured in type
I collagen gels with or without transforming growth factor beta (TGF-?) and/or
triptolide. The diameter of the collagen gel was used to measure contraction.
Immunoblot analysis was used to quantify myosin light chain (MLC) phosphorylation
and integrin expression. Laser confocal fluorescence microscopy was used to
monitor the formation of actin stress fibers. Fibronectin production was measured
with an enzyme immunoassay. RESULTS: Triptolide inhibition of contraction in
TGF-?-induced collagen gel mediated by HTFs was dose-dependent and statistically
significant at 3 nmol/L (P<0.05) and maximal at 30 nmol/L and significantly time
dependent at 2d (P<0.05). Triptolide reduced TGF-?-induced expression of
integrins ?5 and ?1, phosphorylation of MLC, and formation of stress fibers in
HTFs. Furthermore, the inhibition of triptolide on the attenuated TGF-?-induced
production of fibronectin by HTFs was concentration-dependent and significant at
1 nmol/L (P<0.05) and maximal at 30 nmol/L. CONCLUSION: Triptolide suppress the
contractility of HTFs induced by TGF-? and the production of fibronectin by these
cells. It is promising that triptolide treatment may possibly inhibit scar
formation after glaucoma filtration surgery.
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