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10.3389/fcell.2018.00074 http://scihub22266oqcxt.onion/10.3389/fcell.2018.00074 C6048188!6048188 !30042943     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=30042943 &cmd=llinks): Failed to open stream: HTTP request failed! 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Video-Rate Bioluminescence Imaging of Degranulation of Mast Cells Attached to the Extracellular Matrix |pdf=|usr=}}{{30042943 }} gab.com Text Video-Rate Bioluminescence Imaging of Degranulation of Mast Cells Attached to the Extracellular Matrix JO: Front Cell Dev Biol http://www.kidney.de/pget.php?&tw=1&pmid=30042943 #FOAvip Degranulation refers to the secretion of inflammatory mediators, such as histamine, serotonin, and proteases, that are stored within the granules of mast cells and that trigger allergic reactions. The amount of these released mediators has been measured biochemically using cell mass. To investigate degranulation in living single cells, fluorescence microscopy has traditionally been used to observe the disappearance of granules and the appearance of these discharged granules within the plasma membrane by membrane fusion and the movement of granules inside the cells. Here, we developed a method of video-rate bioluminescence imaging to directly detect degranulation from a single mast cell by measuring luminescence activity derived from the enzymatic reaction between Gaussia luciferase (GLase) and its substrate coelenterazine. The neuropeptide Y (NPY), which was reported to colocalize with serotonin in the secretory granules, fused to GLase (NPY-GLase) was efficiently expressed in rat basophilic leukemia (RBL-2H3) cells, a mast-cell line, using a preferred human codon-optimized gene. Bioluminescence imaging analysis of RBL-2H3 cells expressing NPY-GLase and adhered on a glass-bottomed dish showed that the luminescence signals from the resting cells were negligible, while the luminescence signals of the secreted NPY-GLase were repeatedly detected after the addition of an antigen. In addition, this imaging method was applicable for observing degranulation in RBL-2H3 cells that adhered to the extracellular matrix (ECM). These results indicated that video-rate bioluminescence imaging using GLase will be a useful tool for detecting degranulation in single mast cells adhered to a variety of ECM proteins. Twit Text FOAVip Video-Rate Bioluminescence Imaging of Degranulation of Mast Cells Attached to the Extracellular Matrix ????Front Cell Dev Biol http://www.kidney.de/pget.php?&tw=1&pmid=30042943 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=30042943 #FOAvip English Wikipedia <ref>{{Cite pmid|30042943 }}</ref> Video-Rate Bioluminescence Imaging of Degranulation of Mast Cells Attached to the Extracellular Matrix #MMPMID30042943 Yokawa S ; Suzuki T ; Hayashi A ; Inouye S ; Inoh Y ; Furuno T Front Cell Dev Biol 2018[]; 6 (ä): 74 PMID30042943 show ga Degranulation refers to the secretion of inflammatory mediators, such as histamine, serotonin, and proteases, that are stored within the granules of mast cells and that trigger allergic reactions. The amount of these released mediators has been measured biochemically using cell mass. To investigate degranulation in living single cells, fluorescence microscopy has traditionally been used to observe the disappearance of granules and the appearance of these discharged granules within the plasma membrane by membrane fusion and the movement of granules inside the cells. Here, we developed a method of video-rate bioluminescence imaging to directly detect degranulation from a single mast cell by measuring luminescence activity derived from the enzymatic reaction between Gaussia luciferase (GLase) and its substrate coelenterazine. The neuropeptide Y (NPY), which was reported to colocalize with serotonin in the secretory granules, fused to GLase (NPY-GLase) was efficiently expressed in rat basophilic leukemia (RBL-2H3) cells, a mast-cell line, using a preferred human codon-optimized gene. Bioluminescence imaging analysis of RBL-2H3 cells expressing NPY-GLase and adhered on a glass-bottomed dish showed that the luminescence signals from the resting cells were negligible, while the luminescence signals of the secreted NPY-GLase were repeatedly detected after the addition of an antigen. In addition, this imaging method was applicable for observing degranulation in RBL-2H3 cells that adhered to the extracellular matrix (ECM). These results indicated that video-rate bioluminescence imaging using GLase will be a useful tool for detecting degranulation in single mast cells adhered to a variety of ECM proteins. äVideo-Rate Bioluminescence Imaging of Degranulation of Mast Cells Atta(epmc).pdf DeepDyve Pubget Overpricing