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.jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117 Front+Cell+Infect+Microbiol
2018 ; 8
(ä): 235
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The Staphylococcus aureus Extracellular Adherence Protein Eap Is a DNA Binding
Protein Capable of Blocking Neutrophil Extracellular Trap Formation
#MMPMID30038902
Eisenbeis J
; Saffarzadeh M
; Peisker H
; Jung P
; Thewes N
; Preissner KT
; Herrmann M
; Molle V
; Geisbrecht BV
; Jacobs K
; Bischoff M
Front Cell Infect Microbiol
2018[]; 8
(ä): 235
PMID30038902
show ga
The extracellular adherence protein (Eap) of Staphylococcus aureus is a secreted
protein known to exert a number of adhesive and immunomodulatory properties. Here
we describe the intrinsic DNA binding activity of this multifunctional secretory
factor. By using atomic force microscopy, we provide evidence that Eap can bind
and aggregate DNA. While the origin of the DNA substrate (e.g., eukaryotic,
bacterial, phage, and artificial DNA) seems to not be of major importance, the
DNA structure (e.g., linear or circular) plays a critical role with respect to
the ability of Eap to bind and condense DNA. Further functional assays
corroborated the nature of Eap as a DNA binding protein, since Eap suppressed the
formation of "neutrophil extracellular traps" (NETs), composed of DNA-histone
scaffolds, which are thought to function as a neutrophil-mediated extracellular
trapping mechanism. The DNA binding and aggregation activity of Eap may thereby
protect S. aureus against a specific anti-microbial defense reaction from the
host.