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10.2147/IJN.S163149 http://scihub22266oqcxt.onion/10.2147/IJN.S163149 C6045904!6045904 !30022822     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=30022822 &cmd=llinks): Failed to open stream: HTTP request failed! 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Polysaccharide-modified nanoparticles with intelligent CD44 receptor targeting ability for gene delivery |pdf=|usr=}}{{30022822 }} gab.com Text Polysaccharide-modified nanoparticles with intelligent CD44 receptor targeting ability for gene delivery JO: Int J Nanomedicine http://www.kidney.de/pget.php?&tw=1&pmid=30022822 #FOAvip BACKGROUND: Hyaluronic acid (HA) and chondroitin sulfate (CD) are endogenous polysaccharides. In recent years, they have aroused the interest of scientists because of specific binding to CD44 receptors, which are overexpressed in several types of tumors. METHODS: In this study, HA- and CD-modified poly(D,L-lactide-co-glycolide)-poly(ethylene glycol) (PLGA-PEG) copolymers were synthesized and applied to encapsulate 1,2-Dioleoyl-3-trimethylammonium-propane (DOTAP)/pDNA (D/P) lipoplex as CD44 receptor targeting gene delivery nanoparticles (NPs). RESULTS: The particle size of CD-PEG-PLGA-D/P (186.8 ± 21.7 nm) was smaller than that of HA-PEG-PLGA-D/P (270.2 ± 13.8 nm), with narrow size distribution, and both HA-PEG-PLGA-D/P NPs and CD-PEG-PLGA NPs possessed negative zeta potentials (-39.63 ± 5.44 mV and -38.9 ± 2.0 mV, respectively), which prevent erythrocytes from agglutination. Both NPs exhibited pH-dependent release and had faster release in pH 4.0 than in pH 7.4. Generally, the CD-PEG-PLGA-D/P NPs possessed less cytotoxicity than HA-PEG-PLGA-D/P NPs. The D/P-loaded HA-PEG-PLGA and CD-PEG-PLGA NPs expressed significantly higher transfection in CD44 high-expressed U87 (30.1% ± 2.1% and 40.7% ± 4.3%, respectively) than in CD44-negative HepG2 (3.3% ± 1.5% and 1.4% ± 1.0%, respectively) (p < 0.001). It was revealed that the endocytosis of HA-PEG-PLGA-D/P NPs was majorly dominated by macropinocytosis and the endocytosis of CD-PEG-PLGA-D/P NPs was dominated by clathrin-mediated endocytosis pathway (p < 0.001). CONCLUSION: The high selectivity to CD44-positive U87 cancer cells and low cytotoxicity in L929 normal cells assured the promising potential of CD-PEG-PLGA NPs as gene delivery nano-carriers. Twit Text FOAVip Polysaccharide-modified nanoparticles with intelligent CD44 receptor targeting ability for gene delivery ????Int J Nanomedicine http://www.kidney.de/pget.php?&tw=1&pmid=30022822 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=30022822 #FOAvip English Wikipedia <ref>{{Cite pmid|30022822 }}</ref> Polysaccharide-modified nanoparticles with intelligent CD44 receptor targeting ability for gene delivery #MMPMID30022822 Lin WJ ; Lee WC Int J Nanomedicine 2018[]; 13 (ä): 3989-4002 PMID30022822 show ga BACKGROUND: Hyaluronic acid (HA) and chondroitin sulfate (CD) are endogenous polysaccharides. In recent years, they have aroused the interest of scientists because of specific binding to CD44 receptors, which are overexpressed in several types of tumors. METHODS: In this study, HA- and CD-modified poly(D,L-lactide-co-glycolide)-poly(ethylene glycol) (PLGA-PEG) copolymers were synthesized and applied to encapsulate 1,2-Dioleoyl-3-trimethylammonium-propane (DOTAP)/pDNA (D/P) lipoplex as CD44 receptor targeting gene delivery nanoparticles (NPs). RESULTS: The particle size of CD-PEG-PLGA-D/P (186.8 ± 21.7 nm) was smaller than that of HA-PEG-PLGA-D/P (270.2 ± 13.8 nm), with narrow size distribution, and both HA-PEG-PLGA-D/P NPs and CD-PEG-PLGA NPs possessed negative zeta potentials (-39.63 ± 5.44 mV and -38.9 ± 2.0 mV, respectively), which prevent erythrocytes from agglutination. Both NPs exhibited pH-dependent release and had faster release in pH 4.0 than in pH 7.4. Generally, the CD-PEG-PLGA-D/P NPs possessed less cytotoxicity than HA-PEG-PLGA-D/P NPs. The D/P-loaded HA-PEG-PLGA and CD-PEG-PLGA NPs expressed significantly higher transfection in CD44 high-expressed U87 (30.1% ± 2.1% and 40.7% ± 4.3%, respectively) than in CD44-negative HepG2 (3.3% ± 1.5% and 1.4% ± 1.0%, respectively) (p < 0.001). It was revealed that the endocytosis of HA-PEG-PLGA-D/P NPs was majorly dominated by macropinocytosis and the endocytosis of CD-PEG-PLGA-D/P NPs was dominated by clathrin-mediated endocytosis pathway (p < 0.001). CONCLUSION: The high selectivity to CD44-positive U87 cancer cells and low cytotoxicity in L929 normal cells assured the promising potential of CD-PEG-PLGA NPs as gene delivery nano-carriers. |*Gene Transfer Techniques [MESH] |Animals [MESH] |Cell Survival [MESH] |Chondroitin Sulfates/chemistry [MESH] |DNA/metabolism [MESH] |Drug Liberation [MESH] |Endocytosis [MESH] |Erythrocytes/metabolism [MESH] |Fatty Acids, Monounsaturated/chemistry [MESH] |Hep G2 Cells [MESH] |Humans [MESH] |Hyaluronan Receptors/*metabolism [MESH] |Hyaluronic Acid/chemistry [MESH] |Mice [MESH] |Nanoparticles/*chemistry [MESH] |Particle Size [MESH] |Plasmids/metabolism [MESH] |Polyesters/chemistry [MESH] |Polyethylene Glycols/chemistry [MESH] |Polysaccharides/*chemistry [MESH] |Proton Magnetic Resonance Spectroscopy [MESH] |Quaternary Ammonium Compounds/chemistry [MESH]Polysaccharide-modified nanoparticles with intelligent CD44 receptor t(epmc).pdf DeepDyve Pubget Overpricing