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10.1016/bs.mie.2016.09.038 http://scihub22266oqcxt.onion/10.1016/bs.mie.2016.09.038 C6044436!6044436!28062043     free     free     free Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Methods+Enzymol 2017 ; 582 (ä): 387-414 Nephropedia Template TP {{tp|p=28062043|t=2017. Subangstrom measurements of enzyme function using a biological nanopore, SPRNT |pdf=|usr=}}{{28062043}} gab.com Text Subangstrom measurements of enzyme function using a biological nanopore, SPRNT JO: Methods Enzymol http://www.kidney.de/pget.php?&tw=1&pmid=28062043 #FOAvip Nanopores are emerging as new single-molecule tools in the study of enzymes. Based on progress in nanopore sequencing of DNA, a tool called Single-molecule Picometer Resolution Nanopore Tweezers (SPRNT) was developed to measure the movement of enzymes along DNA in real time. In this new method, an enzyme is loaded onto a DNA (or RNA) molecule. A single stranded DNA end of this complex is drawn into a nanopore by an electrostatic potential that is applied across the pore. The single-stranded DNA passes through the pore?s constriction until the enzyme comes into contact with the pore. Further progression of the DNA through the pore is then controlled by the enzyme. An ion current that flows through the pore?s constriction is modulated by the DNA in the constriction. Analysis of ion current changes reveals the advance of the DNA with high spatiotemporal precision, thereby providing a real-time record of the enzyme?s activity. Using an engineered version of the protein nanopore MspA, SPRNT has spatial resolution as small as 40 pm at milliseconds time scales, while simultaneously providing the DNA?s sequence within the enzyme. In this chapter SPRNT is introduced and its extraordinary potential is exemplified using the helicase Hel308. Two distinct sub-states are observed for each one-nucleotide advance; one of these about half-nucleotide long steps is ATP-dependent and the other is ATP-independent. The spatiotemporal resolution of this low-cost single-molecule technique lifts the study of enzymes to a new level of precision, enabling exploration of hitherto unobservable enzyme dynamics in real-time. Twit Text FOAVip Subangstrom measurements of enzyme function using a biological nanopore, SPRNT ????Methods Enzymol http://www.kidney.de/pget.php?&tw=1&pmid=28062043 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28062043 #FOAvip English Wikipedia <ref>{{Cite pmid|28062043}}</ref> Subangstrom measurements of enzyme function using a biological nanopore, SPRNT #MMPMID28062043 Laszlo AH; Derrrington IM; Gundlach JH Methods Enzymol 2017[]; 582 (ä): 387-414 PMID28062043show ga Nanopores are emerging as new single-molecule tools in the study of enzymes. Based on progress in nanopore sequencing of DNA, a tool called Single-molecule Picometer Resolution Nanopore Tweezers (SPRNT) was developed to measure the movement of enzymes along DNA in real time. In this new method, an enzyme is loaded onto a DNA (or RNA) molecule. A single stranded DNA end of this complex is drawn into a nanopore by an electrostatic potential that is applied across the pore. The single-stranded DNA passes through the pore?s constriction until the enzyme comes into contact with the pore. Further progression of the DNA through the pore is then controlled by the enzyme. An ion current that flows through the pore?s constriction is modulated by the DNA in the constriction. Analysis of ion current changes reveals the advance of the DNA with high spatiotemporal precision, thereby providing a real-time record of the enzyme?s activity. Using an engineered version of the protein nanopore MspA, SPRNT has spatial resolution as small as 40 pm at milliseconds time scales, while simultaneously providing the DNA?s sequence within the enzyme. In this chapter SPRNT is introduced and its extraordinary potential is exemplified using the helicase Hel308. Two distinct sub-states are observed for each one-nucleotide advance; one of these about half-nucleotide long steps is ATP-dependent and the other is ATP-independent. The spatiotemporal resolution of this low-cost single-molecule technique lifts the study of enzymes to a new level of precision, enabling exploration of hitherto unobservable enzyme dynamics in real-time. äSubangstrom measurements of enzyme function using a biological nanopor(epmc).pdf DeepDyve Pubget Overpricing