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The effect of tubeimoside-1 on the proliferation, metastasis and apoptosis of
oral squamous cell carcinoma in vitro
#MMPMID30022842
Wu T
; Cui H
; Xu Y
; Du Q
; Zhao E
; Cao J
; Nie L
; Fu G
; Ren A
Onco Targets Ther
2018[]; 11
(?): 3989-4000
PMID30022842
show ga
BACKGROUND: Tubeimoside-1 (TBMS1), a triterpenoid saponin extracted from
traditional Chinese medicine tubeimoside, exerts a cytotoxic effect on several
human cancer cell lines. However, no study has focused on whether TBMS1 works on
oral squamous cell carcinoma (OSCC). MATERIALS AND METHODS: We treated OSCC cells
with TBMS1 to detect the effect and relevant molecular basis of TBMS1 for the
first time. We chose two oral cancer cell lines, CAL27 and SCC15, for this study.
First, the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenylte-trazolium bromide assay
and cell proliferation 5'-bromo-2'-deoxyuridine assay were carried out to detect
cell growth. Second, colony formation assay was performed to assess clonogenesis
capacity. Next apoptosis was analyzed by flow cytometry. Subsequently, wound
healing and transwell assays were applied to explore cell migration. Finally,
Western blot was further performed to examine corresponding proteins' expression
change. RESULTS: Our data showed that TBMS1 significantly suppressed
proliferation of OSCC cells in a dose- and time-dependent manner and it inhibited
migration of OSCC cells as well. After treatment with TBMS1, OSCC cells underwent
cell apoptosis. Furthermore, Western blot demonstrated that TBMS1 downregulated
apoptosis-associated proteins such as PARP, p-ERK1/2, Bcl-2, caspase-3, caspase-7
and caspase-8 and upregulated cleaved PARP, cleaved caspase-3 and cleaved
caspase-9. It could also reduce expression of c-Myc and MMP-7. Meanwhile, TBMS1
did not change the total ERK1/2 expression. CONCLUSION: These results revealed
that TBMS1 might be a potential chemotherapeutic drug for the management of OSCC.
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