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.jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117 J+Stem+Cells+Regen+Med
2018 ; 14
(1
): 53-58
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gab.com Text
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Granulation tissue-derived mesenchymal stromal cells: a potential application for
burn wound healing in pediatric patients
#MMPMID30018473
Pelizzo G
; Avanzini MA
; Mantelli M
; Croce S
; Maltese A
; Vestri E
; De Silvestri A
; Percivalle E
; Calcaterra V
J Stem Cells Regen Med
2018[]; 14
(1
): 53-58
PMID30018473
show ga
Objective: Multipotential cells are mobilized into peripheral blood in response
to trauma, in particular in severe burns. These cells migrate to the site of
injury in response to chemotactic signals to modulate inflammation, repair
damaged tissue and facilitate tissue regeneration. We evaluated the possibility
of isolating and in vitro expand mesenchymal stromal cells (MSCs) from
granulation tissue (GT) during debridement of a burn wound, as a persective
strategy to improve skin regeneration. Methods: GT obtained from a 12-month-old
burn patient was in vitro cultured. Expanded MCSs were characterized for
morphology, immunophenotype, differentiation capacity and proliferative growth.
Antifibrotic features were also evaluated. Results: It was possible to isolate
and in vitro expand cells from GT with the morphology, phenotype, proliferative
and differentiation capacity typical of MSC, these cells were defined as GT-MSC.
GT-MSCs exhibited antifibrotic features by releasing soluble factors, this
activity was superior to that observed in BM-MSC. Conclusions: Successful
isolation and expansion of MSCs from GT is reported. Considering their functional
characteristics, GT-MSCs could be considered a good candidate adjuvant therapy to
improve burn wound healing, particularly in pediatrics.