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10.1073/pnas.1805449115

http://scihub22266oqcxt.onion/10.1073/pnas.1805449115
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C6042120!6042120!29895687
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suck abstract from ncbi

pmid29895687      Proc+Natl+Acad+Sci+U+S+A 2018 ; 115 (26): E5926-33
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  • Microfluidic chambers using fluid walls for cell biology #MMPMID29895687
  • Soitu C; Feuerborn A; Tan AN; Walker H; Walsh PA; Castrejón-Pita AA; Cook PR; Walsh EJ
  • Proc Natl Acad Sci U S A 2018[Jun]; 115 (26): E5926-33 PMID29895687show ga
  • Despite improvements in our ability to manipulate ever-smaller volumes, most workflows in cell biology still use volumes of many microliters. We describe a method for creating microfluidic arrangements containing submicroliter volumes. It exploits interfacial forces dominant at the microscale to confine liquids with fluid (not solid) walls. We demonstrate many basic manipulations required for cell culture and some widely used downstream workflows. The method eliminates many problems associated with the fabrication of conventional microfluidic devices, thereby providing a simple on-demand approach for fabrication of microfluidic devices using materials familiar to biologists.
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