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.jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117 Front+Cell+Infect+Microbiol
2018 ; 8
(ä): 226
Nephropedia Template TP
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Entamoeba histolytica Induce Signaling via Raf/MEK/ERK for Neutrophil
Extracellular Trap (NET) Formation
#MMPMID30023352
Fonseca Z
; Díaz-Godínez C
; Mora N
; Alemán OR
; Uribe-Querol E
; Carrero JC
; Rosales C
Front Cell Infect Microbiol
2018[]; 8
(ä): 226
PMID30023352
show ga
Amoebiasis, the disease caused by Entamoeba histolytica is the third leading
cause of human deaths among parasite infections. E. histolytica was reported
associated with around 100 million cases of amoebic dysentery, colitis and
amoebic liver abscess that lead to almost 50,000 fatalities worldwide in 2010. E.
histolytica infection is associated with the induction of inflammation
characterized by a large number of infiltrating neutrophils. These neutrophils
have been implicated in defense against this parasite, by mechanisms not
completely described. The neutrophil antimicrobial mechanisms include
phagocytosis, degranulation, and formation of neutrophil extracellular traps
(NETs). Recently, our group reported that NETs are also produced in response to
E. histolytica trophozoites. But, the mechanism for NETs induction remains
unknown. In this report we explored the possibility that E. histolytica leads to
NETs formation via a signaling pathway similar to the pathways activated by PMA
or the Fc receptor Fc?RIIIb. Neutrophils were stimulated by E. histolytica
trophozoites and the effect of various pharmacological inhibitors on
amoeba-induced NETs formation was assessed. Selective inhibitors of Raf, MEK, and
NF-?B prevented E. histolytica-induced NET formation. In contrast, inhibitors of
PKC, TAK1, and NADPH-oxidase did not block E. histolytica-induced NETs formation.
E. histolytica induced phosphorylation of ERK in a Raf and MEK dependent manner.
These data show that E. histolytica activates a signaling pathway to induce NETs
formation, that involves Raf/MEK/ERK, but it is independent of PKC, TAK1, and
reactive oxygen species (ROS). Thus, amoebas activate neutrophils via a different
pathway from the pathways activated by PMA or the IgG receptor Fc?RIIIb.
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