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10.1097/MD.0000000000011287 http://scihub22266oqcxt.onion/10.1097/MD.0000000000011287 C6039621!6039621 !29953010     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=29953010 &cmd=llinks): Failed to open stream: HTTP request failed! 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Rapid detection of urinary soluble intercellular adhesion molecule-1 for determination of lupus nephritis activity |pdf=|usr=}}{{29953010 }} gab.com Text Rapid detection of urinary soluble intercellular adhesion molecule-1 for determination of lupus nephritis activity JO: Medicine (Baltimore) http://www.kidney.de/pget.php?&tw=1&pmid=29953010 #FOAvip The current methods of monitoring the activity of lupus nephritis (LN) may cause unnecessary hospital visits or delayed immunosuppressive therapy. We aimed to find a urinary biomarker that could be developed as a home-based test for monitoring the activity of LN.Urine samples were collected immediately before a renal biopsy from patients of suspected active LN, and also from patients with inactive LN, systemic lupus erythematous without LN or healthy controls. Biomarker search was conducted on a cytokine antibody array and confirmation was done by quantitative evaluation with enzyme-linked immunosorbent assay. The Mann-Whiney test or Student t test was used to compare the levels of 9 cytokines between different groups. The sensitivity and specificity of each cytokine for diagnosis of LN was evaluated by receiver operating characteristic curve. A rapid test based on colloidal gold immunochromatography was then developed for bedside or home use. Furthermore, an experimental e-healthcare system was constructed for recording and sharing the results of the rapid test a cloud-assisted internet of things (IoT) consisting of a sensing device, an IoT device and a cloud server.Adiponectin (Acrp30), soluble intercellular cell adhesion molecule-1 (sICAM-1), neural cell adhesion molecule 1 (NCAM-1), and CD26 were significantly higher in urine samples of active LN patients. sICAM-1 appeared more sensitive and specific among these candidates. When the cut-off value of sICAM-1 was set at 1.44?ng/mL, the sensitivity reached 98.33% with a specificity at 85.71%. The sICAM-1 strip test showed comparable sensitivity of 95% and a specificity of 83.3% for assessing the LN activity. Meanwhile, the e-healthcare system was able to conveniently digitize and share the sICAM-1 rapid test results.sICAM-1 appeared to be an excellent biomarker for monitoring LN activity. The e-healthcare system with cloud-assisted IoT could assist the digitalization and sharing of the bedside or home-based sICAM-1 test results. Twit Text FOAVip Rapid detection of urinary soluble intercellular adhesion molecule-1 for determination of lupus nephritis activity ????Medicine (Baltimore) http://www.kidney.de/pget.php?&tw=1&pmid=29953010 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=29953010 #FOAvip English Wikipedia <ref>{{Cite pmid|29953010 }}</ref> Rapid detection of urinary soluble intercellular adhesion molecule-1 for determination of lupus nephritis activity #MMPMID29953010 Wang Y ; Tao Y ; Liu Y ; Zhao Y ; Song C ; Zhou B ; Wang T ; Gao L ; Zhang L ; Hu H Medicine (Baltimore) 2018[Jun]; 97 (26 ): e11287 PMID29953010 show ga The current methods of monitoring the activity of lupus nephritis (LN) may cause unnecessary hospital visits or delayed immunosuppressive therapy. We aimed to find a urinary biomarker that could be developed as a home-based test for monitoring the activity of LN.Urine samples were collected immediately before a renal biopsy from patients of suspected active LN, and also from patients with inactive LN, systemic lupus erythematous without LN or healthy controls. Biomarker search was conducted on a cytokine antibody array and confirmation was done by quantitative evaluation with enzyme-linked immunosorbent assay. The Mann-Whiney test or Student t test was used to compare the levels of 9 cytokines between different groups. The sensitivity and specificity of each cytokine for diagnosis of LN was evaluated by receiver operating characteristic curve. A rapid test based on colloidal gold immunochromatography was then developed for bedside or home use. Furthermore, an experimental e-healthcare system was constructed for recording and sharing the results of the rapid test a cloud-assisted internet of things (IoT) consisting of a sensing device, an IoT device and a cloud server.Adiponectin (Acrp30), soluble intercellular cell adhesion molecule-1 (sICAM-1), neural cell adhesion molecule 1 (NCAM-1), and CD26 were significantly higher in urine samples of active LN patients. sICAM-1 appeared more sensitive and specific among these candidates. When the cut-off value of sICAM-1 was set at 1.44?ng/mL, the sensitivity reached 98.33% with a specificity at 85.71%. The sICAM-1 strip test showed comparable sensitivity of 95% and a specificity of 83.3% for assessing the LN activity. Meanwhile, the e-healthcare system was able to conveniently digitize and share the sICAM-1 rapid test results.sICAM-1 appeared to be an excellent biomarker for monitoring LN activity. The e-healthcare system with cloud-assisted IoT could assist the digitalization and sharing of the bedside or home-based sICAM-1 test results. |Adiponectin/immunology/urine [MESH] |Adult [MESH] |Aged [MESH] |Biomarkers [MESH] |CD56 Antigen/immunology/urine [MESH] |Dipeptidyl Peptidase 4/immunology/urine [MESH] |Enzyme-Linked Immunosorbent Assay [MESH] |Female [MESH] |Humans [MESH] |Intercellular Adhesion Molecule-1/immunology/*urine [MESH] |Lupus Erythematosus, Systemic/immunology/urine [MESH] |Lupus Nephritis/*immunology/*urine [MESH] |Male [MESH] |Middle Aged [MESH]Rapid detection of urinary soluble intercellular adhesion molecule-1 f(epmc).pdf DeepDyve Pubget Overpricing