Evaluation of the analytical performances of Cobas 6500 and Sysmex UN series
automated urinalysis systems with manual microscopic particle counting
#MMPMID30022887
Bakan E
; Bayraktutan Z
; Baygutalp NK
; Gul MA
; Umudum FZ
; Bakan N
Biochem Med (Zagreb)
2018[Jun]; 28
(2
): 020712
PMID30022887
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INTRODUCTION: Automated urinalysis systems are valuable tools in clinical
laboratories, especially those with a high work load. The objective of this study
was to compare the analytical performance of Sysmex UN series urine analyser,
which may become a new one in our laboratory, with the Cobas 6500 automated urine
analyser, which is used in our laboratory for a long time. For comparisons,
manual microscopical examination was accepted as reference method. MATERIALS AND
METHODS: A total of 470 urine samples were tested in the two automated urinalysis
systems, and urine sediment testing with manual microscopy was applied to a 100
pathological samples of the total 470. The diagnostic performance of the two
automated urine analysers was compared with each other and manual microscopy.
RESULTS: Differences were determined between automated and manual microscopy in
some pathological samples. The resultant regression equations were as follows.
Comparison of Cobas U701 with Sysmex UF-5000: y = - 0.57 (- 0.85 to - 0.29) +
0.95 (0.92 to 0.99) x for RBC, and y = - 0.11 (- 0.54 to 0.29) + 0.89 (0.84 to
0.98) x for WBC; comparison of Cobas U701 with manual microscopy: y = - 0.45 (-
0.85 to 0.21) + 1.00 (0.92 to 1.07) x for WBC; and comparison of Sysmex UF-5000
with manual microscopy: y = - 0.74 (- 1.09 to - 0.57) + 0.87 (0.85 to 0.91) x for
WBC. CONCLUSIONS: We can conclude that the new Sysmex UN series urine analyser
can be safely used in our laboratory. Although the results showed good to
moderate concordance, the microscopy results of the automated platforms should be
confirmed by manual microscopy, particularly in pathological samples.
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